6QZ3

Structure of MHETase from Ideonella sakaiensis

6QZ3 の概要

• エントリーDOI: 10.2210/pdb6qz3/pdb
• 分子名称: Mono(2-hydroxyethyl) terephthalate hydrolase, BENZOIC ACID, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: mhetase, pet degradation, structural genomics
• 由来する生物種: Ideonella sakaiensis (strain 201-F6)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 65321.85

構造登録者

Allen, M.D.,Johnson, C.W.,Knott, B.C.,Beckham, G.T.,McGeehan, J.E. (登録日: 2019-03-11, 公開日: 2020-09-30, 最終更新日: 2024-10-16)

主引用文献

Knott, B.C.,Erickson, E.,Allen, M.D.,Gado, J.E.,Graham, R.,Kearns, F.L.,Pardo, I.,Topuzlu, E.,Anderson, J.J.,Austin, H.P.,Dominick, G.,Johnson, C.W.,Rorrer, N.A.,Szostkiewicz, C.J.,Copie, V.,Payne, C.M.,Woodcock, H.L.,Donohoe, B.S.,Beckham, G.T.,McGeehan, J.E.
Characterization and engineering of a two-enzyme system for plastics depolymerization.
Proc.Natl.Acad.Sci.USA, 117:25476-25485, 2020

PubMed Abstract: Plastics pollution represents a global environmental crisis. In response, microbes are evolving the capacity to utilize synthetic polymers as carbon and energy sources. Recently, was reported to secrete a two-enzyme system to deconstruct polyethylene terephthalate (PET) to its constituent monomers. Specifically, the PETase depolymerizes PET, liberating soluble products, including mono(2-hydroxyethyl) terephthalate (MHET), which is cleaved to terephthalic acid and ethylene glycol by MHETase. Here, we report a 1.6 Å resolution MHETase structure, illustrating that the MHETase core domain is similar to PETase, capped by a lid domain. Simulations of the catalytic itinerary predict that MHETase follows the canonical two-step serine hydrolase mechanism. Bioinformatics analysis suggests that MHETase evolved from ferulic acid esterases, and two homologous enzymes are shown to exhibit MHET turnover. Analysis of the two homologous enzymes and the MHETase S131G mutant demonstrates the importance of this residue for accommodation of MHET in the active site. We also demonstrate that the MHETase lid is crucial for hydrolysis of MHET and, furthermore, that MHETase does not turnover mono(2-hydroxyethyl)-furanoate or mono(2-hydroxyethyl)-isophthalate. A highly synergistic relationship between PETase and MHETase was observed for the conversion of amorphous PET film to monomers across all nonzero MHETase concentrations tested. Finally, we compare the performance of MHETase:PETase chimeric proteins of varying linker lengths, which all exhibit improved PET and MHET turnover relative to the free enzymes. Together, these results offer insights into the two-enzyme PET depolymerization system and will inform future efforts in the biological deconstruction and upcycling of mixed plastics.

PubMed: 32989159
DOI: 10.1073/pnas.2006753117

実験手法

X-RAY DIFFRACTION (1.6 Å)