6QYZ

The cryo-EM structure of prohead RNA in bacteriophage phi29 prohead

6QYZ の概要

• エントリーDOI: 10.2210/pdb6qyz/pdb
• EMDBエントリー: 4655 4662 4680
• 分子名称: the prohead RNA (71-MER) in bacteriophage phi29 (1 entity in total)
• 機能のキーワード: bacteriophage, phi29, prohead, virus
• 由来する生物種: Bacillus phage phi29
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 192092.89

構造登録者

Xu, J.,Wang, D.,Gui, M.,Xiang, Y. (登録日: 2019-03-10, 公開日: 2019-06-12, 最終更新日: 2024-05-15)

主引用文献

Xu, J.,Wang, D.,Gui, M.,Xiang, Y.
Structural assembly of the tailed bacteriophage φ29.
Nat Commun, 10:2366-2366, 2019

PubMed Abstract: The mature virion of the tailed bacteriophage ϕ29 is an ~33 MDa complex that contains more than 450 subunits of seven structural proteins assembling into a prolate head and a short non-contractile tail. Here, we report the near-atomic structures of the ϕ29 pre-genome packaging head (prohead), the mature virion and the genome-emptied virion. Structural comparisons suggest local rotation or oscillation of the head-tail connector upon DNA packaging and release. Termination of the DNA packaging occurs through pressure-dependent correlative positional and conformational changes in the connector. The funnel-shaped tail lower collar attaches the expanded narrow end of the connector and has a 180-Å long, 24-strand β barrel narrow stem tube that undergoes conformational changes upon genome release. The appendages form an interlocked assembly attaching the tail around the collar. The membrane active long loops at the distal end of the tail knob exit during the late stage of infection and form the cone-shaped tip of a largely hydrophobic helix barrel, prepared for membrane penetration.

PubMed: 31147544
DOI: 10.1038/s41467-019-10272-3

実験手法

ELECTRON MICROSCOPY (4.6 Å)