6QY2

Pink beam serial crystallography: Lysozyme, 5 us exposure, 750 patterns merged

Summary for 6QY2

• Entry DOI: 10.2210/pdb6qy2/pdb
• Related: 6QXV 6QXW 6QXX 6QXY 6QY0 6QY1
• Descriptor: Lysozyme C, SODIUM ION, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: pink beam, fixed-target, serial crystallography, lysozyme, hydrolase
• Biological source: Gallus gallus (Chicken)
• Total number of polymer chains: 1
• Total formula weight: 14510.11

Authors

Lieske, J.,Tolstikova, A.,Meents, A. (deposition date: 2019-03-08, release date: 2019-09-25, Last modification date: 2024-10-16)

Primary citation

Tolstikova, A.,Levantino, M.,Yefanov, O.,Hennicke, V.,Fischer, P.,Meyer, J.,Mozzanica, A.,Redford, S.,Crosas, E.,Opara, N.L.,Barthelmess, M.,Lieske, J.,Oberthuer, D.,Wator, E.,Mohacsi, I.,Wulff, M.,Schmitt, B.,Chapman, H.N.,Meents, A.
1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector.
Iucrj, 6:927-937, 2019

PubMed Abstract: Reliable sample delivery and efficient use of limited beam time have remained bottlenecks for serial crystallography (SX). Using a high-intensity polychromatic X-ray beam in combination with a newly developed charge-integrating JUNGFRAU detector, we have applied the method of fixed-target SX to collect data at a rate of 1 kHz at a synchrotron-radiation facility. According to our data analysis for the given experimental conditions, only about 3 000 diffraction patterns are required for a high-quality diffraction dataset. With indexing rates of up to 25%, recording of such a dataset takes less than 30 s.

PubMed: 31576225
DOI: 10.1107/S205225251900914X

Experimental method

X-RAY DIFFRACTION (1.7 Å)