6QW3

Calcium-bound gelsolin domain 2

6QW3 の概要

• エントリーDOI: 10.2210/pdb6qw3/pdb
• 分子名称: Gelsolin, CALCIUM ION (3 entities in total)
• 機能のキーワード: amyloidosis, gelsolin, actin-binding, structural protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13206.73

構造登録者

Scalone, E.,Boni, F.,Milani, M.,Mastrangelo, E.,de Rosa, M. (登録日: 2019-03-05, 公開日: 2019-08-28, 最終更新日: 2024-11-13)

主引用文献

Bollati, M.,Scalone, E.,Boni, F.,Mastrangelo, E.,Giorgino, T.,Milani, M.,de Rosa, M.
High-resolution crystal structure of gelsolin domain 2 in complex with the physiological calcium ion.
Biochem.Biophys.Res.Commun., 518:94-99, 2019

PubMed Abstract: The second domain of gelsolin (G2) hosts mutations responsible for a hereditary form of amyloidosis. The active form of gelsolin is Ca-bound; it is also a dynamic protein, hence structural biologists often rely on the study of the isolated G2. However, the wild type G2 structure that have been used so far in comparative studies is bound to a crystallographic Cd, in lieu of the physiological calcium. Here, we report the wild type structure of G2 in complex with Ca highlighting subtle ion-dependent differences. Previous findings on different G2 mutations are also briefly revised in light of these results.

PubMed: 31416615
DOI: 10.1016/j.bbrc.2019.08.013

実験手法

X-RAY DIFFRACTION (1.3 Å)