6QVJ

HsCKK (human CAMSAP1) decorated 14pf taxol-GDP microtubule

6QVJ の概要

• エントリーDOI: 10.2210/pdb6qvj/pdb
• 関連するPDBエントリー: 6QUS 6QUY
• EMDBエントリー: 4643 4644 4654
• 分子名称: Calmodulin-regulated spectrin-associated protein 1, Tubulin alpha-1B chain, Tubulin beta chain, ... (7 entities in total)
• 機能のキーワード: microtubule camsap calmodulin-regulated spectrum-associated proteins ckk cryo-em cryo-electron microscopy, structural protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 223162.00

構造登録者

Atherton, J.M.,Luo, Y.,Xiang, S.,Yang, C.,Jiang, K.,Stangier, M.,Vemu, A.,Cook, A.,Wang, S.,Roll-Mecak, A.,Steinmetz, M.O.,Akhmanova, A.,Baldus, M.,Moores, C.A. (登録日: 2019-03-02, 公開日: 2019-11-27, 最終更新日: 2024-05-15)

主引用文献

Atherton, J.,Luo, Y.,Xiang, S.,Yang, C.,Rai, A.,Jiang, K.,Stangier, M.,Vemu, A.,Cook, A.D.,Wang, S.,Roll-Mecak, A.,Steinmetz, M.O.,Akhmanova, A.,Baldus, M.,Moores, C.A.
Structural determinants of microtubule minus end preference in CAMSAP CKK domains.
Nat Commun, 10:5236-5236, 2019

PubMed Abstract: CAMSAP/Patronins regulate microtubule minus-end dynamics. Their end specificity is mediated by their CKK domains, which we proposed recognise specific tubulin conformations found at minus ends. To critically test this idea, we compared the human CAMSAP1 CKK domain (HsCKK) with a CKK domain from Naegleria gruberi (NgCKK), which lacks minus-end specificity. Here we report near-atomic cryo-electron microscopy structures of HsCKK- and NgCKK-microtubule complexes, which show that these CKK domains share the same protein fold, bind at the intradimer interprotofilament tubulin junction, but exhibit different footprints on microtubules. NMR experiments show that both HsCKK and NgCKK are remarkably rigid. However, whereas NgCKK binding does not alter the microtubule architecture, HsCKK remodels its microtubule interaction site and changes the underlying polymer structure because the tubulin lattice conformation is not optimal for its binding. Thus, in contrast to many MAPs, the HsCKK domain can differentiate subtly specific tubulin conformations to enable microtubule minus-end recognition.

PubMed: 31748546
DOI: 10.1038/s41467-019-13247-6

実験手法

ELECTRON MICROSCOPY (3.8 Å)