6QUJ

GHK tagged GFP variant

6QUJ の概要

• エントリーDOI: 10.2210/pdb6quj/pdb
• 関連するPDBエントリー: 6QUG 6QUH 6QUI
• 分子名称: Green fluorescent protein, COPPER (II) ION, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: gfp, fluorescent protein
• 由来する生物種: Aequorea victoria
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 53266.17

構造登録者

Huyton, T.,Gorlich, D. (登録日: 2019-02-27, 公開日: 2020-05-27, 最終更新日: 2024-10-09)

主引用文献

Mehr, A.,Henneberg, F.,Chari, A.,Gorlich, D.,Huyton, T.
The copper(II)-binding tripeptide GHK, a valuable crystallization and phasing tag for macromolecular crystallography.
Acta Crystallogr D Struct Biol, 76:1222-1232, 2020

PubMed Abstract: The growth of diffraction-quality crystals and experimental phasing remain two of the main bottlenecks in protein crystallography. Here, the high-affinity copper(II)-binding tripeptide GHK was fused to the N-terminus of a GFP variant and an MBP-FG peptide fusion. The GHK tag promoted crystallization, with various residues (His, Asp, His/Pro) from symmetry molecules completing the copper(II) square-pyramidal coordination sphere. Rapid structure determination by copper SAD phasing could be achieved, even at a very low Bijvoet ratio or after significant radiation damage. When collecting highly redundant data at a wavelength close to the copper absorption edge, residual S-atom positions could also be located in log-likelihood-gradient maps and used to improve the phases. The GHK copper SAD method provides a convenient way of both crystallizing and phasing macromolecular structures, and will complement the current trend towards native sulfur SAD and MR-SAD phasing.

PubMed: 33263328
DOI: 10.1107/S2059798320013741

実験手法

X-RAY DIFFRACTION (1.68 Å)