6QU8

Adenovirus Serotype 26 (Ad26) in complex with sialic acid, pH8.0

6QU8 の概要

• エントリーDOI: 10.2210/pdb6qu8/pdb
• 分子名称: Fiber, N-acetyl-alpha-neuraminic acid, N-acetyl-beta-neuraminic acid, ... (5 entities in total)
• 機能のキーワード: adenovirus, adenovirus 26, ad26, hadv-d26, ad26k, hadv-d26k, human adenovirus, human adenovirus 26, mastadenovirus, sialic acid, nana, sia, n-acetylneuraminic acid, neuraminic acid, species d, adenovirus species d, add, hadv-d, protein iv, piv, fiber-knob, fiber, head domain, knob domain, fiber protein, fibre protein, fibre-knob, knob, head, viral protein
• 由来する生物種: Human adenovirus 26
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24834.59

構造登録者

Baker, A.T.,Rizkallah, P.J.,Parker, A.L.,Mundy, R.M. (登録日: 2019-02-26, 公開日: 2019-09-18, 最終更新日: 2024-01-24)

主引用文献

Baker, A.T.,Mundy, R.M.,Davies, J.A.,Rizkallah, P.J.,Parker, A.L.
Human adenovirus type 26 uses sialic acid-bearing glycans as a primary cell entry receptor.
Sci Adv, 5:eaax3567-eaax3567, 2019

PubMed Abstract: Adenoviruses are clinically important agents. They cause respiratory distress, gastroenteritis, and epidemic keratoconjunctivitis. As non-enveloped, double-stranded DNA viruses, they are easily manipulated, making them popular vectors for therapeutic applications, including vaccines. Species D adenovirus type 26 (HAdV-D26) is both a cause of EKC and other diseases and a promising vaccine vector. HAdV-D26-derived vaccines are under investigation as protective platforms against HIV, Zika, and respiratory syncytial virus infections and are in phase 3 clinical trials for Ebola. We recently demonstrated that HAdV-D26 does not use CD46 or Desmoglein-2 as entry receptors, while the putative interaction with coxsackie and adenovirus receptor is low affinity and unlikely to represent the primary cell receptor. Here, we establish sialic acid as a primary entry receptor used by HAdV-D26. We demonstrate that removal of cell surface sialic acid inhibits HAdV-D26 infection, and provide a high-resolution crystal structure of HAdV-D26 fiber-knob in complex with sialic acid.

PubMed: 31517055
DOI: 10.1126/sciadv.aax3567

実験手法

X-RAY DIFFRACTION (1.19 Å)