6QTZ の概要
| エントリーDOI | 10.2210/pdb6qtz/pdb |
| EMDBエントリー | 10068 10071 4560 4630 4636 4884 |
| 分子名称 | 25S rRNA, 60S ribosomal protein L13-A, 60S ribosomal protein L23-A, ... (49 entities in total) |
| 機能のキーワード | 60s subunit, eif6, nmd3, lsg1, ribosome |
| 由来する生物種 | Saccharomyces cerevisiae 詳細 |
| タンパク質・核酸の鎖数 | 48 |
| 化学式量合計 | 2154021.66 |
| 構造登録者 | |
| 主引用文献 | Kargas, V.,Castro-Hartmann, P.,Escudero-Urquijo, N.,Dent, K.,Hilcenko, C.,Sailer, C.,Zisser, G.,Marques-Carvalho, M.J.,Pellegrino, S.,Wawiorka, L.,Freund, S.M.,Wagstaff, J.L.,Andreeva, A.,Faille, A.,Chen, E.,Stengel, F.,Bergler, H.,Warren, A.J. Mechanism of completion of peptidyltransferase centre assembly in eukaryotes. Elife, 8:-, 2019 Cited by PubMed Abstract: During their final maturation in the cytoplasm, pre-60S ribosomal particles are converted to translation-competent large ribosomal subunits. Here, we present the mechanism of peptidyltransferase centre (PTC) completion that explains how integration of the last ribosomal proteins is coupled to release of the nuclear export adaptor Nmd3. Single-particle cryo-EM reveals that eL40 recruitment stabilises helix 89 to form the uL16 binding site. The loading of uL16 unhooks helix 38 from Nmd3 to adopt its mature conformation. In turn, partial retraction of the L1 stalk is coupled to a conformational switch in Nmd3 that allows the uL16 P-site loop to fully accommodate into the PTC where it competes with Nmd3 for an overlapping binding site (base A2971). Our data reveal how the central functional site of the ribosome is sculpted and suggest how the formation of translation-competent 60S subunits is disrupted in leukaemia-associated ribosomopathies. PubMed: 31115337DOI: 10.7554/eLife.44904 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3.5 Å) |
構造検証レポート
検証レポート(詳細版)
をダウンロード
をダウンロード
