6QTF

Solution NMR of synthetic analogues of nisin and mutacin ring A and ring B - Mutacin I Ring B, major conformer

6QTF の概要

• エントリーDOI: 10.2210/pdb6qtf/pdb
• 関連するPDBエントリー: 1WCO 6QM1
• NMR情報: BMRB: 34363
• 分子名称: DCY-LEU-GLY-ALA-THR (1 entity in total)
• 機能のキーワード: peptide antibiotic, lantibiotic, antimicrobial, bacteriocin, thioester, antibiotic
• 由来する生物種: Lactococcus lactis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 463.55

構造登録者

Dickman, R.,Mitchell, S.A.,Figueiredo, A.,Hansen, D.F.,Tabor, A.B. (登録日: 2019-02-25, 公開日: 2019-09-11, 最終更新日: 2024-10-16)

主引用文献

Dickman, R.,Mitchell, S.A.,Figueiredo, A.M.,Hansen, D.F.,Tabor, A.B.
Molecular Recognition of Lipid II by Lantibiotics: Synthesis and Conformational Studies of Analogues of Nisin and Mutacin Rings A and B.
J.Org.Chem., 84:11493-11512, 2019

PubMed Abstract: In response to the growing threat posed by antibiotic-resistant bacterial strains, extensive research is currently focused on developing antimicrobial agents that target lipid II, a vital precursor in the biosynthesis of bacterial cell walls. The lantibiotic nisin and related peptides display unique and highly selective binding to lipid II. A key feature of the nisin-lipid II interaction is the formation of a cage-like complex between the pyrophosphate moiety of lipid II and the two thioether-bridged rings, rings A and B, at the N-terminus of nisin. To understand the important structural factors underlying this highly selective molecular recognition, we have used solid-phase peptide synthesis to prepare individual ring A and B structures from nisin, the related lantibiotic mutacin, and synthetic analogues. Through NMR studies of these rings, we have demonstrated that ring A is preorganized to adopt the correct conformation for binding lipid II in solution and that individual amino acid substitutions in ring A have little effect on the conformation. We have also analyzed the turn structures adopted by these thioether-bridged peptides and show that they do not adopt the tight α-turn or β-turn structures typically found in proteins.

PubMed: 31464129
DOI: 10.1021/acs.joc.9b01253

実験手法

SOLUTION NMR