6QSP

Ketosynthase (ApeO) in Complex with its Chain Length Factor (ApeC) from Xenorhabdus doucetiae

6QSP の概要

• エントリーDOI: 10.2210/pdb6qsp/pdb
• 関連するPDBエントリー: 1TQY 2IWZ
• 分子名称: Beta-ketoacyl synthase, Uncharacterized protein, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: aryl polyene pigment, biosynthesis, type ii polyketide synthase, ketoacyl synthase, chain elongation, claisen condensation, biosynthetic protein
• 由来する生物種: Xenorhabdus doucetiae; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 70555.76

構造登録者

Grammbitter, G.L.C.,Schmalhofer, M.,Groll, M.,Bode, H. (登録日: 2019-02-21, 公開日: 2019-04-03, 最終更新日: 2024-01-24)

主引用文献

Grammbitter, G.L.C.,Schmalhofer, M.,Karimi, K.,Shi, Y.M.,Schoner, T.A.,Tobias, N.J.,Morgner, N.,Groll, M.,Bode, H.B.
An Uncommon Type II PKS Catalyzes Biosynthesis of Aryl Polyene Pigments.
J.Am.Chem.Soc., 141:16615-16623, 2019

PubMed Abstract: Aryl polyene (APE) pigments are a widely distributed class of bacterial polyketides. So far, little is known about the biosynthesis of these compounds, which are produced by a novel type II polyketide synthase (PKS). We have identified all enzymes involved in APE biosynthesis and determined their peculiar functions. The biosynthesis was reconstituted , and ACP-bound intermediates were assigned for each reaction step by HPLC-MS. Native mass spectrometry experiments identified four stable complexes: the acyl-carrier proteins ApeE and ApeF bound to the thioesterase ApeK, the dehydratases ApeI and ApeP, and the ketosynthase ApeO in complex with its chain-length factor ApeC. X-ray structures of the heterodimeric ApeO:ApeC and ApeI:ApeP complexes depict striking protein-protein interactions. Altogether, our study elucidated mechanistic aspects of APE biosynthesis that unifies elements of type II fatty acid and PKS systems, but in addition includes novel enzyme complexes.

PubMed: 30908039
DOI: 10.1021/jacs.8b10776

実験手法

X-RAY DIFFRACTION (1.45 Å)