6QPB

Cryo-EM structure of calcium-free mTMEM16F lipid scramblase in digitonin

6QPB の概要

• エントリーDOI: 10.2210/pdb6qpb/pdb
• EMDBエントリー: 4612
• 分子名称: Anoctamin-6, 1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE (2 entities in total)
• 機能のキーワード: lipid scrambles, tmem16, membrane protein
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 213868.91

構造登録者

Alvadia, C.,Lim, N.K.,Clerico Mosina, V.,Oostergetel, G.T.,Dutzler, R.,Paulino, C. (登録日: 2019-02-13, 公開日: 2019-03-06, 最終更新日: 2024-11-13)

主引用文献

Alvadia, C.,Lim, N.K.,Clerico Mosina, V.,Oostergetel, G.T.,Dutzler, R.,Paulino, C.
Cryo-EM structures and functional characterization of the murine lipid scramblase TMEM16F.
Elife, 8:-, 2019

PubMed Abstract: The lipid scramblase TMEM16F initiates blood coagulation by catalyzing the exposure of phosphatidylserine in platelets. The protein is part of a family of membrane proteins, which encompasses calcium-activated channels for ions and lipids. Here, we reveal features of murine TMEM16F (mTMEM16F) that underlie its function as a lipid scramblase and an ion channel. The cryo-EM data of mTMEM16F in absence and presence of Ca define the ligand-free closed conformation of the protein and the structure of a Ca-bound intermediate. Both conformations resemble their counterparts of the scrambling-incompetent anion channel mTMEM16A, yet with distinct differences in the region of ion and lipid permeation. In conjunction with functional data, we demonstrate the relationship between ion conduction and lipid scrambling. Although activated by a common mechanism, both functions appear to be mediated by alternate protein conformations that are at equilibrium in the ligand-bound state.

PubMed: 30785399
DOI: 10.7554/eLife.44365

実験手法

ELECTRON MICROSCOPY (3.6 Å)