6QJZ
Identificationand Characterization of an Oxalylfrom Grass pea (Lathyrus sativusCoA-Synthetase L.)
Summary for 6QJZ
| Entry DOI | 10.2210/pdb6qjz/pdb |
| Descriptor | oxalyl CoA-synthetase, ADENOSINE MONOPHOSPHATE (3 entities in total) |
| Functional Keywords | adenylating anl superfamily, ligase |
| Biological source | Lathyrus sativus |
| Total number of polymer chains | 1 |
| Total formula weight | 56509.07 |
| Authors | |
| Primary citation | Goldsmith, M.,Barad, S.,Peleg, Y.,Albeck, S.,Dym, O.,Brandis, A.,Mehlman, T.,Reich, Z. The identification and characterization of an oxalyl-CoA synthetase from grass pea ( Lathyrus sativus L.). Rsc Chem Biol, 3:320-333, 2022 Cited by PubMed Abstract: Oxalic acid is a small metabolite found in many plants. It serves as protection from herbivores, a chelator of metal ions, a regulator of calcium levels, and additional tasks. However, it is also a strong di-carboxylic acid that can compromise plant viability by reducing cellular pH. Several metabolic pathways have evolved to control oxalate levels in plants by enzymatic degradation. Among them is the pathway that utilizes oxalyl-CoA synthetase (OCS, EC 6.2.1.8) and ATP to convert oxalate to oxalyl-CoA. Oxalyl-CoA can then be degraded to CO or utilized as a precursor for the synthesis of other compounds. In grass pea ( L.), a grain legume grown in Asia and Africa for human and animal consumption, the neurotoxic compound β--oxalyl-l-α,β-diaminopropionic acid (β-ODAP) is synthesized from oxalyl-CoA and l-α,β-diaminopropionic acid (l-DAPA). Here, we report on the identification and characterization of oxalyl CoA-synthetase from grass pea (OCS). The gene encoding OCS was amplified from grass pea, and then expressed and purified from cells as an untagged, monomeric protein of 56 kDa. Its catalytic efficiency with oxalate, = 71.5 ± 13.3 μM, = 8.2 ± 0.8 μmole min mg, was similar to that of OCS homologs from (AAE3) (AAE3). The enzyme was crystalized in complex with AMP and is the first OCS whose structure was determined in the thioester-forming conformation. Finally, we propose that substituting OCS with an oxalate oxidase or decarboxylase may reduce the levels of β-ODAP in grass pea. PubMed: 35359497DOI: 10.1039/d1cb00202c PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
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