6QEQ

PcfF from Enterococcus faecalis pCF10

6QEQ の概要

• エントリーDOI: 10.2210/pdb6qeq/pdb
• 分子名称: PcfF (2 entities in total)
• 機能のキーワード: accessory factor, dna binding protein
• 由来する生物種: Enterococcus faecalis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 56327.23

構造登録者

Rehman, S.,Berntsson, R.P.A. (登録日: 2019-01-08, 公開日: 2019-05-01, 最終更新日: 2024-11-20)

主引用文献

Rehman, S.,Li, Y.G.,Schmitt, A.,Lassinantti, L.,Christie, P.J.,Berntsson, R.P.
Enterococcal PcfF Is a Ribbon-Helix-Helix Protein That Recruits the Relaxase PcfG Through Binding and Bending of the oriT Sequence.
Front Microbiol, 10:958-958, 2019

PubMed Abstract: The conjugative plasmid pCF10 from encodes a Type 4 Secretion System required for plasmid transfer. The accessory factor PcfF and relaxase PcfG initiate pCF10 transfer by forming the catalytically active relaxosome at the plasmid's origin-of-transfer () sequence. Here, we report the crystal structure of the homo-dimeric PcfF, composed of an N-terminal DNA binding Ribbon-Helix-Helix (RHH) domain and a C-terminal stalk domain. We identified key residues in the RHH domain that are responsible for binding pCF10's sequence , and further showed that PcfF bends the DNA upon binding. By mutational analysis and pull-down experiments, we identified residues in the stalk domain that contribute to interaction with PcfG. PcfF variant proteins defective in or PcfG binding attenuated plasmid transfer , but also suggested that intrinsic or extrinsic factors might modulate relaxosome assembly. We propose that PcfF initiates relaxosome assembly by binding and inducing DNA bending, which serves to recruit PcfG as well as extrinsic factors necessary for optimal plasmid processing and engagement with the pCF10 transfer machine.

PubMed: 31134011
DOI: 10.3389/fmicb.2019.00958

実験手法

X-RAY DIFFRACTION (1.9 Å)