6QD4

MloK1 model from single particle analysis of 2D crystals, class 8 (intermediate conformation)

6QD4 の概要

• エントリーDOI: 10.2210/pdb6qd4/pdb
• 関連するPDBエントリー: 6I9D 6IAX 6QCY 6QCZ 6QD0 6QD1 6QD2 6QD3
• EMDBエントリー: 4519
• 分子名称: Cyclic nucleotide-gated potassium channel mll3241, POTASSIUM ION (2 entities in total)
• 機能のキーワード: voltage-gated potassium channel, cyclic nucleotide-binding domain, ion channel, ion transport, membrane protein
• 由来する生物種: Mesorhizobium loti MAFF303099
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 151143.38

構造登録者

Righetto, R.,Biyani, N.,Kowal, J.,Chami, M.,Stahlberg, H. (登録日: 2018-12-31, 公開日: 2019-04-24, 最終更新日: 2024-05-15)

主引用文献

Righetto, R.D.,Biyani, N.,Kowal, J.,Chami, M.,Stahlberg, H.
Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM.
Nat Commun, 10:1722-1722, 2019

PubMed Abstract: Electron crystallography can reveal the structure of membrane proteins within 2D crystals under close-to-native conditions. High-resolution structural information can only be reached if crystals are perfectly flat and highly ordered. In practice, such crystals are difficult to obtain. Available image unbending algorithms correct for disorder, but only perform well on images of non-tilted, flat crystals, while out-of-plane distortions are not addressed. Here, we present an approach that employs single-particle refinement procedures to locally unbend crystals in 3D. With this method, density maps of the MloK1 potassium channel with a resolution of 4 Å were obtained from images of 2D crystals that do not diffract beyond 10 Å. Furthermore, 3D classification allowed multiple structures to be resolved, revealing a series of MloK1 conformations within a single 2D crystal. This conformational heterogeneity explains the poor diffraction observed and is related to channel function. The approach is implemented in the FOCUS package.

PubMed: 30979902
DOI: 10.1038/s41467-019-09661-5

実験手法

ELECTRON MICROSCOPY (5.6 Å)