6QBE

Crystal structure of non-toxic HaNLP3 protein

6QBE の概要

• エントリーDOI: 10.2210/pdb6qbe/pdb
• 分子名称: Nep1-like protein, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: non-toxic nlp protein, beta-sandwich, hyaloperonospora arabidopsidis, toxin
• 由来する生物種: Hyaloperonospora arabidopsidis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29285.22

構造登録者

Lenarcic, T.,Podobnik, M.,Anderluh, G. (登録日: 2018-12-21, 公開日: 2019-08-28, 最終更新日: 2024-01-24)

主引用文献

Lenarcic, T.,Pirc, K.,Hodnik, V.,Albert, I.,Borisek, J.,Magistrato, A.,Nurnberger, T.,Podobnik, M.,Anderluh, G.
Molecular basis for functional diversity among microbial Nep1-like proteins.
Plos Pathog., 15:e1007951-e1007951, 2019

PubMed Abstract: Necrosis and ethylene-inducing peptide 1 (Nep1)-like proteins (NLPs) are secreted by several phytopathogenic microorganisms. They trigger necrosis in various eudicot plants upon binding to plant sphingolipid glycosylinositol phosphorylceramides (GIPC). Interestingly, HaNLP3 from the obligate biotroph oomycete Hyaloperonospora arabidopsidis does not induce necrosis. We determined the crystal structure of HaNLP3 and showed that it adopts the NLP fold. However, the conformations of the loops surrounding the GIPC headgroup-binding cavity differ from those of cytotoxic Pythium aphanidermatum NLPPya. Essential dynamics extracted from μs-long molecular dynamics (MD) simulations reveals a limited conformational plasticity of the GIPC-binding cavity in HaNLP3 relative to toxic NLPs. This likely precludes HaNLP3 binding to GIPCs, which is the underlying reason for the lack of toxicity. This study reveals that mutations at key protein regions cause a switch between non-toxic and toxic phenotypes within the same protein scaffold. Altogether, these data provide evidence that protein flexibility is a distinguishing trait of toxic NLPs and highlight structural determinants for a potential functional diversification of non-toxic NLPs utilized by biotrophic plant pathogens.

PubMed: 31479498
DOI: 10.1371/journal.ppat.1007951

実験手法

X-RAY DIFFRACTION (2 Å)