6Q64

BT1044SeMet E190Q

6Q64 の概要

• エントリーDOI: 10.2210/pdb6q64/pdb
• 分子名称: Endoglycosidase (1 entity in total)
• 機能のキーワード: glycoside hydrolase family 18, complex n-glycans, hydrolase
• 由来する生物種: Bacteroides thetaiotaomicron
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42117.87

構造登録者

Basle, A.,Paterson, N.,Crouch, L.,Bolam, D. (登録日: 2018-12-10, 公開日: 2019-05-08, 最終更新日: 2024-11-20)

主引用文献

Briliute, J.,Urbanowicz, P.A.,Luis, A.S.,Basle, A.,Paterson, N.,Rebello, O.,Hendel, J.,Ndeh, D.A.,Lowe, E.C.,Martens, E.C.,Spencer, D.I.R.,Bolam, D.N.,Crouch, L.I.
Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci.
Nat Microbiol, 4:1571-1581, 2019

PubMed Abstract: Glycans are the major carbon sources available to the human colonic microbiota. Numerous N-glycosylated proteins are found in the human gut, from both dietary and host sources, including immunoglobulins such as IgA that are secreted into the intestine at high levels. Here, we show that many mutualistic gut Bacteroides spp. have the capacity to utilize complex N-glycans (CNGs) as nutrients, including those from immunoglobulins. Detailed mechanistic studies using transcriptomic, biochemical, structural and genetic techniques reveal the pathway employed by Bacteroides thetaiotaomicron (Bt) for CNG degradation. The breakdown process involves an extensive enzymatic apparatus encoded by multiple non-adjacent loci and comprises 19 different carbohydrate-active enzymes from different families, including a CNG-specific endo-glycosidase activity. Furthermore, CNG degradation involves the activity of carbohydrate-active enzymes that have previously been implicated in the degradation of other classes of glycan. This complex and diverse apparatus provides Bt with the capacity to access the myriad different structural variants of CNGs likely to be found in the intestinal niche.

PubMed: 31160824
DOI: 10.1038/s41564-019-0466-x

実験手法

X-RAY DIFFRACTION (2.4 Å)