6Q4W

Structure of MPT-1, a GDP-Man-dependent mannosyltransferase from Leishmania mexicana

6Q4W の概要

• エントリーDOI: 10.2210/pdb6q4w/pdb
• 分子名称: LmxM MPT-1 (2 entities in total)
• 機能のキーワード: transferase
• 由来する生物種: Leishmania mexicana (strain MHOM/GT/2001/U1103)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 78010.16

構造登録者

Sobala, L.F.,Males, A.,Bastidas, L.M.,Ward, T.,Sernee, M.F.,Ralton, J.E.,Nero, T.L.,Cobbold, S.,Kloehn, J.,Viera-Lara, M.,Stanton, L.,Hanssen, E.,Parker, M.W.,Williams, S.J.,McConville, M.J.,Davies, G.J. (登録日: 2018-12-06, 公開日: 2019-09-18, 最終更新日: 2024-01-24)

主引用文献

Sernee, M.F.,Ralton, J.E.,Nero, T.L.,Sobala, L.F.,Kloehn, J.,Vieira-Lara, M.A.,Cobbold, S.A.,Stanton, L.,Pires, D.E.V.,Hanssen, E.,Males, A.,Ward, T.,Bastidas, L.M.,van der Peet, P.L.,Parker, M.W.,Ascher, D.B.,Williams, S.J.,Davies, G.J.,McConville, M.J.
A Family of Dual-Activity Glycosyltransferase-Phosphorylases Mediates Mannogen Turnover and Virulence in Leishmania Parasites.
Cell Host Microbe, 26:385-399.e9, 2019

PubMed Abstract: Parasitic protists belonging to the genus Leishmania synthesize the non-canonical carbohydrate reserve, mannogen, which is composed of β-1,2-mannan oligosaccharides. Here, we identify a class of dual-activity mannosyltransferase/phosphorylases (MTPs) that catalyze both the sugar nucleotide-dependent biosynthesis and phosphorolytic turnover of mannogen. Structural and phylogenic analysis shows that while the MTPs are structurally related to bacterial mannan phosphorylases, they constitute a distinct family of glycosyltransferases (GT108) that have likely been acquired by horizontal gene transfer from gram-positive bacteria. The seven MTPs catalyze the constitutive synthesis and turnover of mannogen. This metabolic rheostat protects obligate intracellular parasite stages from nutrient excess, and is essential for thermotolerance and parasite infectivity in the mammalian host. Our results suggest that the acquisition and expansion of the MTP family in Leishmania increased the metabolic flexibility of these protists and contributed to their capacity to colonize new host niches.

PubMed: 31513773
DOI: 10.1016/j.chom.2019.08.009

実験手法

X-RAY DIFFRACTION (1.55 Å)