6Q4S

Crystal structure of a-eudesmol synthase

6Q4S の概要

• エントリーDOI: 10.2210/pdb6q4s/pdb
• 分子名称: Pentalenene synthase, CHLORIDE ION (3 entities in total)
• 機能のキーワード: sesquiterpene, terpene synthase, plant protein, unknown function
• 由来する生物種: Streptomyces chartreusis NRRL 3882
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39534.18

構造登録者

Correia Cordeiro, R.S.,Hakansson, M.,Logan, D.T.,Kourist, R. (登録日: 2018-12-06, 公開日: 2018-12-19, 最終更新日: 2024-01-24)

主引用文献

Kracht, O.N.,Correia Cordeiro, R.S.,Hakansson, M.,Stockmann, J.,Sander, D.,Bandow, J.,Senges, C.H.R.,Logan, D.T.,Kourist, R.
Discovery of three novel sesquiterpene synthases from Streptomyces chartreusis NRRL 3882 and crystal structure of an alpha-eudesmol synthase.
J.Biotechnol., 297:71-77, 2019

PubMed Abstract: With more than 50,000 members, terpenoids are one of the most important classes of natural products and show an enormous diversity. Due to their unique odors and specific bioactivities they already find wide application in the flavor, fragrance and pharma industries. Since most terpenoids can only be obtained by natural product extraction, the discovery of biosynthetic genes for the generation of terpene diversity becomes increasingly important. This study describes the discovery of three novel sesquiterpene synthases from Streptomyces chartreusis with preference for the formation of germacradiene-11-ol, α-eudesmol and α-amorphene respectively. The α-eudesmol synthase showed formation of 10-epi-δ-eudesmol and elemol as side products. Eudesmol-isomers are known to have repellent activity, which makes this enzyme a potential catalyst for products for the prevention of mosquito-related disease. The determination of the structure of the apo-enzyme of α-eudesmol synthase from S. chartreusis provides the first structural insights into an eudesmol-forming enzyme.

PubMed: 30928538
DOI: 10.1016/j.jbiotec.2019.03.006

実験手法

X-RAY DIFFRACTION (1.83 Å)