6Q1F

Atomic structure of the Human Herpesvirus 6B Capsid and Capsid-Associated Tegument Complexes

This is a non-PDB format compatible entry.

Summary for 6Q1F

• Entry DOI: 10.2210/pdb6q1f/pdb
• EMDB information: 20557 20558 20559 20560
• Descriptor: Major capsid protein, Large structural phosphoprotein, Small capsomere-interacting protein, ... (5 entities in total)
• Functional Keywords: beta-herpesvirus, hhv-6b, murine cytomegalovirus, human cytomegalovirus, pp150, pu11, pul32, pm32, pu14, virus
• Biological source: Human herpesvirus 6B (strain Z29) (HHV-6 variant B); More
• Total number of polymer chains: 59
• Total formula weight: 4248211.38

Authors

Zhang, Y.B.,Liu, W.,Li, Z.H.,Kumar, V.,Alvarez-Cabrera, A.L.,Leibovitch, E.,Cui, Y.X.,Mei, Y.,Bi, G.Q.,Jacobson, S.,Zhou, Z.H. (deposition date: 2019-08-03, release date: 2019-12-11, Last modification date: 2024-03-20)

Primary citation

Zhang, Y.,Liu, W.,Li, Z.,Kumar, V.,Alvarez-Cabrera, A.L.,Leibovitch, E.C.,Cui, Y.,Mei, Y.,Bi, G.Q.,Jacobson, S.,Zhou, Z.H.
Atomic structure of the human herpesvirus 6B capsid and capsid-associated tegument complexes.
Nat Commun, 10:5346-5346, 2019

PubMed Abstract: Human herpesvirus 6B (HHV-6B) belongs to the β-herpesvirus subfamily of the Herpesviridae. To understand capsid assembly and capsid-tegument interactions, here we report atomic structures of HHV-6B capsid and capsid-associated tegument complex (CATC) obtained by cryoEM and sub-particle reconstruction. Compared to other β-herpesviruses, HHV-6B exhibits high similarity in capsid structure but organizational differences in its CATC (pU11 tetramer). 180 "VΛ"-shaped CATCs are observed in HHV-6B, distinguishing from the 255 "Λ"-shaped dimeric CATCs observed in murine cytomegalovirus and the 310 "Δ"-shaped CATCs in human cytomegalovirus. This trend in CATC quantity correlates with the increasing genomes sizes of these β-herpesviruses. Incompatible distances revealed by the atomic structures rationalize the lack of CATC's binding to triplexes Ta, Tc, and Tf in HHV-6B. Our results offer insights into HHV-6B capsid assembly and the roles of its tegument proteins, including not only the β-herpesvirus-specific pU11 and pU14, but also those conserved across all subfamilies of Herpesviridae.

PubMed: 31767868
DOI: 10.1038/s41467-019-13064-x

Experimental method

ELECTRON MICROSCOPY (9 Å)