6Q0B

Poliovirus (Type 1 Mahoney), receptor-catalysed 135S particle incubated with anti-VP1 mAb at RT for 1 hr

6Q0B の概要

• エントリーDOI: 10.2210/pdb6q0b/pdb
• EMDBエントリー: 20546
• 分子名称: Capsid protein VP1, Capsid protein VP2, Capsid protein VP3, ... (5 entities in total)
• 機能のキーワード: poliovirus, cell-entry intermediate, expanded virus, a-particle, virus-immune system complex, virus/immune system
• 由来する生物種: Poliovirus type 1 (strain Mahoney); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 142255.34

構造登録者

Hogle, J.M.,Filman, D.J.,Shah, P.N.M. (登録日: 2019-08-01, 公開日: 2020-08-05, 最終更新日: 2022-12-21)

主引用文献

Shah, P.N.M.,Filman, D.J.,Karunatilaka, K.S.,Hesketh, E.L.,Groppelli, E.,Strauss, M.,Hogle, J.M.
Cryo-EM structures reveal two distinct conformational states in a picornavirus cell entry intermediate.
Plos Pathog., 16:e1008920-e1008920, 2020

PubMed Abstract: The virions of enteroviruses such as poliovirus undergo a global conformational change after binding to the cellular receptor, characterized by a 4% expansion, and by the opening of holes at the two and quasi-three-fold symmetry axes of the capsid. The resultant particle is called a 135S particle or A-particle and is thought to be on the pathway to a productive infection. Previously published studies have concluded that the membrane-interactive peptides, namely VP4 and the N-terminus of VP1, are irreversibly externalized in the 135S particle. However, using established protocols to produce the 135S particle, and single particle cryo-electron microscopy methods, we have identified at least two unique states that we call the early and late 135S particle. Surprisingly, only in the "late" 135S particles have detectable levels of the VP1 N-terminus been trapped outside the capsid. Moreover, we observe a distinct density inside the capsid that can be accounted for by VP4 that remains associated with the genome. Taken together our results conclusively demonstrate that the 135S particle is not a unique conformation, but rather a family of conformations that could exist simultaneously.

PubMed: 32997730
DOI: 10.1371/journal.ppat.1008920

実験手法

ELECTRON MICROSCOPY (3.4 Å)