6PWG

Ewingella americana HopBF1 kinase bound to AMP-PNP

Summary for 6PWG

• Entry DOI: 10.2210/pdb6pwg/pdb
• Related: 6PWD
• Descriptor: Type III effector HopBF1, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, 1,2-ETHANEDIOL, ... (4 entities in total)
• Functional Keywords: hsp90, phosphorylation, chaperone, immunity, kinase, transferase
• Biological source: Ewingella americana
• Total number of polymer chains: 2
• Total formula weight: 46523.38

Authors

Tomchick, D.R.,Tagliabracci, V.S.,Park, B.C. (deposition date: 2019-07-23, release date: 2019-09-11, Last modification date: 2023-10-11)

Primary citation

Lopez, V.A.,Park, B.C.,Nowak, D.,Sreelatha, A.,Zembek, P.,Fernandez, J.,Servage, K.A.,Gradowski, M.,Hennig, J.,Tomchick, D.R.,Pawlowski, K.,Krzymowska, M.,Tagliabracci, V.S.
A Bacterial Effector Mimics a Host HSP90 Client to Undermine Immunity.
Cell, 179:205-218.e21, 2019

PubMed Abstract: The molecular chaperone HSP90 facilitates the folding of several client proteins, including innate immune receptors and protein kinases. HSP90 is an essential component of plant and animal immunity, yet pathogenic strategies that directly target the chaperone have not been described. Here, we identify the HopBF1 family of bacterial effectors as eukaryotic-specific HSP90 protein kinases. HopBF1 adopts a minimal protein kinase fold that is recognized by HSP90 as a host client. As a result, HopBF1 phosphorylates HSP90 to completely inhibit the chaperone's ATPase activity. We demonstrate that phosphorylation of HSP90 prevents activation of immune receptors that trigger the hypersensitive response in plants. Consequently, HopBF1-dependent phosphorylation of HSP90 is sufficient to induce severe disease symptoms in plants infected with the bacterial pathogen, Pseudomonas syringae. Collectively, our results uncover a family of bacterial effector kinases with toxin-like properties and reveal a previously unrecognized betrayal mechanism by which bacterial pathogens modulate host immunity.

PubMed: 31522888
DOI: 10.1016/j.cell.2019.08.020

Experimental method

X-RAY DIFFRACTION (1.89 Å)