6PWE

Cryo-EM structure of nucleosome core particle

6PWE の概要

• エントリーDOI: 10.2210/pdb6pwe/pdb
• EMDBエントリー: 20506 20507
• 分子名称: Histone H3, Histone H4, Histone H2A, ... (6 entities in total)
• 機能のキーワード: nucleosome, histone, dna, dna-binding protein, structural protein-dna complex, structural protein/dna
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 198639.50

構造登録者

Chittori, S.,Subramaniam, S. (登録日: 2019-07-22, 公開日: 2019-08-21, 最終更新日: 2024-03-20)

主引用文献

Chittori, S.,Hong, J.,Bai, Y.,Subramaniam, S.
Structure of the primed state of the ATPase domain of chromatin remodeling factor ISWI bound to the nucleosome.
Nucleic Acids Res., 47:9400-9409, 2019

PubMed Abstract: ATP-dependent chromatin remodeling factors of SWI/SNF2 family including ISWI, SNF2, CHD1 and INO80 subfamilies share a conserved but functionally non-interchangeable ATPase domain. Here we report cryo-electron microscopy (cryo-EM) structures of the nucleosome bound to an ISWI fragment with deletion of the AutoN and HSS regions in nucleotide-free conditions and the free nucleosome at ∼ 4 Å resolution. In the bound conformation, the ATPase domain interacts with the super helical location 2 (SHL 2) of the nucleosomal DNA, with the N-terminal tail of H4 and with the α1 helix of H3. Density for other regions of ISWI is not observed, presumably due to disorder. Comparison with the structure of the free nucleosome reveals that although the histone core remains largely unchanged, remodeler binding causes perturbations in the nucleosomal DNA resulting in a bulge near the SHL2 site. Overall, the structure of the nucleotide-free ISWI-nucleosome complex is similar to the corresponding regions of the recently reported ADP bound ISWI-nucleosome structures, which are significantly different from that observed for the ADP-BeFx bound structure. Our findings are relevant to the initial step of ISWI binding to the nucleosome and provide additional insights into the nucleosome remodeling process driven by ISWI.

PubMed: 31402386
DOI: 10.1093/nar/gkz670

実験手法

ELECTRON MICROSCOPY (3.95 Å)