6PWD

Ewingella americana HopBF1 kinase

6PWD の概要

• エントリーDOI: 10.2210/pdb6pwd/pdb
• 分子名称: Type III effector HopBF1, 1,2-ETHANEDIOL, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: hsp90, phosphorylation, chaperone, immunity, kinase, transferase
• 由来する生物種: Ewingella americana
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23068.79

構造登録者

Tomchick, D.R.,Tagliabracci, V.S.,Park, B.C. (登録日: 2019-07-22, 公開日: 2019-09-11, 最終更新日: 2024-03-13)

主引用文献

Lopez, V.A.,Park, B.C.,Nowak, D.,Sreelatha, A.,Zembek, P.,Fernandez, J.,Servage, K.A.,Gradowski, M.,Hennig, J.,Tomchick, D.R.,Pawlowski, K.,Krzymowska, M.,Tagliabracci, V.S.
A Bacterial Effector Mimics a Host HSP90 Client to Undermine Immunity.
Cell, 179:205-218.e21, 2019

PubMed Abstract: The molecular chaperone HSP90 facilitates the folding of several client proteins, including innate immune receptors and protein kinases. HSP90 is an essential component of plant and animal immunity, yet pathogenic strategies that directly target the chaperone have not been described. Here, we identify the HopBF1 family of bacterial effectors as eukaryotic-specific HSP90 protein kinases. HopBF1 adopts a minimal protein kinase fold that is recognized by HSP90 as a host client. As a result, HopBF1 phosphorylates HSP90 to completely inhibit the chaperone's ATPase activity. We demonstrate that phosphorylation of HSP90 prevents activation of immune receptors that trigger the hypersensitive response in plants. Consequently, HopBF1-dependent phosphorylation of HSP90 is sufficient to induce severe disease symptoms in plants infected with the bacterial pathogen, Pseudomonas syringae. Collectively, our results uncover a family of bacterial effector kinases with toxin-like properties and reveal a previously unrecognized betrayal mechanism by which bacterial pathogens modulate host immunity.

PubMed: 31522888
DOI: 10.1016/j.cell.2019.08.020

実験手法

X-RAY DIFFRACTION (2.47 Å)