6PUP

1.9 A crystal structure of flavodoxin-like domain of Schizosaccharomyces japonicus putative tRNAPhe 4-demethylwyosine synthase Tyw1 in complex with FMN

6PUP の概要

• エントリーDOI: 10.2210/pdb6pup/pdb
• 分子名称: Wybutosine biosynthesis protein Tyw1, MANGANESE (II) ION, FLAVIN MONONUCLEOTIDE, ... (4 entities in total)
• 機能のキーワード: trna, hypermodification, wyosine, metal binding, flavoprotein
• 由来する生物種: Schizosaccharomyces japonicus (strain yFS275 / FY16936) (Fission yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20316.60

構造登録者

Sjekloca, L.,Ferre D'Amare, A.R. (登録日: 2019-07-18, 公開日: 2020-07-29, 最終更新日: 2024-05-22)

主引用文献

Sjekloca, L.,Ferre-D'Amare, A.R.
Biochemical and structural characterization of the flavodoxin-like domain of the Schizosaccharomyces japonicus putative tRNAPhe 4-demethylwyosine synthase Tyw1 in complex with FMN.
MicroPubl Biol, 2022:-, 2022

PubMed Abstract: The S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 catalyzes biosynthesis of 4-demethylwyosine (imG-14), the precursor for wyosine, the hypermodified guanine-derived nucleotide present at position 37 of phenylalanine tRNAs of archaea and eukarya. Eukaryotic TYW1 enzymes contain N-terminal flavodoxin-like and C-terminal radical-SAM domains. We determined co-crystal structures of the flavodoxin-like domain of the putative Tyw1 from in complex with flavin mononucleotide (FMN), exploiting an unexpected anomalous scatterer present in the recombinant protein. Our results show how eukaryotic TYW1 enzymes bind the coenzyme FMN and will help further elucidation of the structural enzymology of 4-demethylwyosine synthesis.

PubMed: 35693892
DOI: 10.17912/micropub.biology.000570

実験手法

X-RAY DIFFRACTION (1.89 Å)