6PSS

Escherichia coli RNA polymerase promoter unwinding intermediate (TRPi1.5a) with TraR and mutant rpsT P2 promoter

6PSS の概要

• エントリーDOI: 10.2210/pdb6pss/pdb
• EMDBエントリー: 20462
• 分子名称: DNA-directed RNA polymerase subunit alpha, ZINC ION, DNA-directed RNA polymerase subunit beta, ... (10 entities in total)
• 機能のキーワード: transcription, transcription-dna complex, transcription/dna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 560386.09

構造登録者

Chen, J.,Chiu, C.E.,Campbell, E.A.,Darst, S.A. (登録日: 2019-07-13, 公開日: 2020-03-25, 最終更新日: 2024-03-20)

主引用文献

Chen, J.,Chiu, C.,Gopalkrishnan, S.,Chen, A.Y.,Olinares, P.D.B.,Saecker, R.M.,Winkelman, J.T.,Maloney, M.F.,Chait, B.T.,Ross, W.,Gourse, R.L.,Campbell, E.A.,Darst, S.A.
Stepwise Promoter Melting by Bacterial RNA Polymerase.
Mol.Cell, 78:275-, 2020

PubMed Abstract: Transcription initiation requires formation of the open promoter complex (RPo). To generate RPo, RNA polymerase (RNAP) unwinds the DNA duplex to form the transcription bubble and loads the DNA into the RNAP active site. RPo formation is a multi-step process with transient intermediates of unknown structure. We use single-particle cryoelectron microscopy to visualize seven intermediates containing Escherichia coli RNAP with the transcription factor TraR en route to forming RPo. The structures span the RPo formation pathway from initial recognition of the duplex promoter in a closed complex to the final RPo. The structures and supporting biochemical data define RNAP and promoter DNA conformational changes that delineate steps on the pathway, including previously undetected transient promoter-RNAP interactions that contribute to populating the intermediates but do not occur in RPo. Our work provides a structural basis for understanding RPo formation and its regulation, a major checkpoint in gene expression throughout evolution.

PubMed: 32160514
DOI: 10.1016/j.molcel.2020.02.017

実験手法

ELECTRON MICROSCOPY (3.5 Å)