6PNS

In situ structure of BTV RNA-dependent RNA polymerase in BTV virion

6PNS の概要

• エントリーDOI: 10.2210/pdb6pns/pdb
• EMDBエントリー: 20398
• 分子名称: RNA-directed RNA polymerase, Inner core structural protein VP3 (2 entities in total)
• 機能のキーワード: rna dependent rna polymerase, viral protein, transferase
• 由来する生物種: Bluetongue virus 1 (BTV-1); 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 1184031.36

構造登録者

He, Y.,Shivakoti, S.,Ding, K.,Cui, Y.,Roy, P.,Zhou, Z.H. (登録日: 2019-07-03, 公開日: 2019-08-07, 最終更新日: 2025-05-28)

主引用文献

He, Y.,Shivakoti, S.,Ding, K.,Cui, Y.,Roy, P.,Zhou, Z.H.
In situ structures of RNA-dependent RNA polymerase inside bluetongue virus before and after uncoating.
Proc.Natl.Acad.Sci.USA, 116:16535-16540, 2019

PubMed Abstract: Bluetongue virus (BTV), a major threat to livestock, is a multilayered, nonturreted member of the , a family of segmented dsRNA viruses characterized by endogenous RNA transcription through an RNA-dependent RNA polymerase (RdRp). To date, the structure of BTV RdRp has been unknown, limiting our mechanistic understanding of BTV transcription and hindering rational drug design effort targeting this essential enzyme. Here, we report the in situ structures of BTV RdRp VP1 in both the triple-layered virion and double-layered core, as determined by cryo-electron microscopy (cryoEM) and subparticle reconstruction. BTV RdRp has 2 unique motifs not found in other viral RdRps: a fingernail, attached to the conserved fingers subdomain, and a bundle of 3 helices: 1 from the palm subdomain and 2 from the N-terminal domain. BTV RdRp VP1 is anchored to the inner surface of the capsid shell via 5 asymmetrically arranged N termini of the inner capsid shell protein VP3A around the 5-fold axis. The structural changes of RdRp VP1 and associated capsid shell proteins between BTV virions and cores suggest that the detachment of the outer capsid proteins VP2 and VP5 during viral entry induces both global movements of the inner capsid shell and local conformational changes of the N-terminal latch helix (residues 34 to 51) of 1 inner capsid shell protein VP3A, priming RdRp VP1 within the capsid for transcription. Understanding this mechanism in BTV also provides general insights into RdRp activation and regulation during viral entry of other multilayered, nonturreted dsRNA viruses.

PubMed: 31350350
DOI: 10.1073/pnas.1905849116

実験手法

ELECTRON MICROSCOPY (3.7 Å)