6PL9
Adduct formed after 1 month in the reaction of dichlorido(1,3-dimethylbenzimidaz ol-2-ylidene)(eta5-pentamethylcyclopentadienyl)rhodium(III) with HEWL
Summary for 6PL9
| Entry DOI | 10.2210/pdb6pl9/pdb |
| Descriptor | Lysozyme, dichloro[(1,2,3,4,5-eta)-pentamethylcyclopentadienyl]rhodium, 2-(1-chloranyl-2,3,4,5,6-pentamethyl-1$l^{7}-rhodapentacyclo[2.2.0.0^{1,3}.0^{1,5}.0^{2,6}]hexan-1-yl)-1,3-dimethyl-benzimidazole, ... (5 entities in total) |
| Functional Keywords | metal-based, anticancer, ruthenium, nhc, carbene, lysozyme, metallodrug, benzimidazole, dimethylbenzimidazole, hydrolase |
| Biological source | Gallus gallus (Chicken) |
| Total number of polymer chains | 1 |
| Total formula weight | 15082.96 |
| Authors | Sullivan, M.P.,Hartinger, C.G.,Goldstone, D.C. (deposition date: 2019-06-30, release date: 2020-11-25, Last modification date: 2024-10-23) |
| Primary citation | Sullivan, M.P.,Cziferszky, M.,Tolbatov, I.,Truong, D.,Mercadante, D.,Re, N.,Gust, R.,Goldstone, D.C.,Hartinger, C. Probing the Paradigm of Promiscuity for N-Heterocyclic Carbene Complexes and their Protein Adduct Formation. Angew.Chem.Int.Ed.Engl., 2021 Cited by PubMed Abstract: Metal complexes can be considered a "paradigm of promiscuity" when it comes to their interactions with proteins. They often form adducts with a variety of donor atoms in an unselective manner. We have characterized the adducts formed between a series of isostructural N-heterocyclic carbene (NHC) complexes with Ru, Os, Rh, and Ir centers and the model protein hen egg white lysozyme by X-ray crystallography and mass spectrometry. Distinctive behavior for the metal compounds was observed with the more labile Ru and Rh complexes targeting mainly a surface l-histidine moiety through cleavage of p-cymene or NHC co-ligands, respectively. In contrast, the more inert Os and Ir derivatives were detected abundantly in an electronegative binding pocket after undergoing ligand exchange of a chlorido ligand for an amino acid side chain. Computational studies supported the binding profiles and hinted at the role of the protein microenvironment for metal complexes eliciting selectivity for specific binding sites on the protein. PubMed: 34196088DOI: 10.1002/anie.202106906 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.2 Å) |
Structure validation
Download full validation report
