6PGK

Membrane Protein Megahertz Crystallography at the European XFEL, Photosystem I XFEL at 2.9 A

6PGK の概要

• エントリーDOI: 10.2210/pdb6pgk/pdb
• 分子名称: Photosystem I P700 chlorophyll a apoprotein A1, Photosystem I reaction center subunit XI, Photosystem I reaction center subunit XII, ... (21 entities in total)
• 機能のキーワード: photosynthesis, light harvesting, photosystem i
• 由来する生物種: Thermosynechococcus elongatus (strain BP-1); 詳細
• タンパク質・核酸の鎖数: 36
• 化学式量合計: 1080124.27

構造登録者

Fromme, R.,Gisriel, C.,Fromme, P. (登録日: 2019-06-24, 公開日: 2019-11-27, 最終更新日: 2024-11-20)

主引用文献

Gisriel, C.,Coe, J.,Letrun, R.,Yefanov, O.M.,Luna-Chavez, C.,Stander, N.E.,Lisova, S.,Mariani, V.,Kuhn, M.,Aplin, S.,Grant, T.D.,Dorner, K.,Sato, T.,Echelmeier, A.,Cruz Villarreal, J.,Hunter, M.S.,Wiedorn, M.O.,Knoska, J.,Mazalova, V.,Roy-Chowdhury, S.,Yang, J.H.,Jones, A.,Bean, R.,Bielecki, J.,Kim, Y.,Mills, G.,Weinhausen, B.,Meza, J.D.,Al-Qudami, N.,Bajt, S.,Brehm, G.,Botha, S.,Boukhelef, D.,Brockhauser, S.,Bruce, B.D.,Coleman, M.A.,Danilevski, C.,Discianno, E.,Dobson, Z.,Fangohr, H.,Martin-Garcia, J.M.,Gevorkov, Y.,Hauf, S.,Hosseinizadeh, A.,Januschek, F.,Ketawala, G.K.,Kupitz, C.,Maia, L.,Manetti, M.,Messerschmidt, M.,Michelat, T.,Mondal, J.,Ourmazd, A.,Previtali, G.,Sarrou, I.,Schon, S.,Schwander, P.,Shelby, M.L.,Silenzi, A.,Sztuk-Dambietz, J.,Szuba, J.,Turcato, M.,White, T.A.,Wrona, K.,Xu, C.,Abdellatif, M.H.,Zook, J.D.,Spence, J.C.H.,Chapman, H.N.,Barty, A.,Kirian, R.A.,Frank, M.,Ros, A.,Schmidt, M.,Fromme, R.,Mancuso, A.P.,Fromme, P.,Zatsepin, N.A.
Membrane protein megahertz crystallography at the European XFEL.
Nat Commun, 10:5021-5021, 2019

PubMed Abstract: The world's first superconducting megahertz repetition rate hard X-ray free-electron laser (XFEL), the European XFEL, began operation in 2017, featuring a unique pulse train structure with 886 ns between pulses. With its rapid pulse rate, the European XFEL may alleviate some of the increasing demand for XFEL beamtime, particularly for membrane protein serial femtosecond crystallography (SFX), leveraging orders-of-magnitude faster data collection. Here, we report the first membrane protein megahertz SFX experiment, where we determined a 2.9 Å-resolution SFX structure of the large membrane protein complex, Photosystem I, a > 1 MDa complex containing 36 protein subunits and 381 cofactors. We address challenges to megahertz SFX for membrane protein complexes, including growth of large quantities of crystals and the large molecular and unit cell size that influence data collection and analysis. The results imply that megahertz crystallography could have an important impact on structure determination of large protein complexes with XFELs.

PubMed: 31685819
DOI: 10.1038/s41467-019-12955-3

実験手法

X-RAY DIFFRACTION (2.9 Å)