6PFZ

Structure of a NAD-Dependent Persulfide Reductase from A. fulgidus

6PFZ の概要

• エントリーDOI: 10.2210/pdb6pfz/pdb
• 分子名称: NADH oxidase (NoxA-3), FLAVIN-ADENINE DINUCLEOTIDE, COENZYME A, ... (5 entities in total)
• 機能のキーワード: nadh-dependent reductase, persulfide reductase, disulfide reductase, polysulfide reductase, flavoprotein
• 由来する生物種: Archaeoglobus fulgidus DSM 4304
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 247388.90

構造登録者

Sazinsky, M.H.,Shabdar, S.,Garcia-Constineiras, A.,Crane III, E.J. (登録日: 2019-06-23, 公開日: 2020-07-01, 最終更新日: 2023-10-11)

主引用文献

Shabdar, S.,Anaclet, B.,Castineiras, A.G.,Desir, N.,Choe, N.,Crane 3rd, E.J.,Sazinsky, M.H.
Structural and Kinetic Characterization of Hyperthermophilic NADH-Dependent Persulfide Reductase from Archaeoglobus fulgidus .
Archaea, 2021:8817136-8817136, 2021

PubMed Abstract: NADH-dependent persulfide reductase (Npsr) has been proposed to facilitate dissimilatory sulfur respiration by reducing persulfide or sulfane sulfur-containing substrates to HS. The presence of this gene in the sulfate and thiosulfate-reducing DSM 4304 and other hyperthermophilic appears anomalous, as is unable to respire S and grow in the presence of elemental sulfur. To assess the role of Npsr in the sulfur metabolism of DSM 4304, the Npsr from was characterized. AfNpsr is specific for persulfide and polysulfide as substrates in the oxidative half-reaction, exhibiting / on the order of 10 M s, which is similar to the kinetic parameters observed for hyperthermophilic CoA persulfide reductases. In contrast to the bacterial Npsr, AfNpsr exhibits low disulfide reductase activity with DTNB; however, similar to the bacterial enzymes, it does not show detectable activity with CoA-disulfide, oxidized glutathione, or cystine. The 3.1 Å X-ray structure of AfNpsr reveals access to the tightly bound catalytic CoA, and the active site Cys 42 is restricted by a flexible loop (residues 60-66) that is not seen in the bacterial homologs from and . Unlike the bacterial enzymes, AfNpsr exhibits NADH oxidase activity and also shows no detectable activity with NADPH. Models suggest steric and electrostatic repulsions of the NADPH 2'-phosphate account for the strong preference for NADH. The presence of Npsr in the nonsulfur-reducing suggests that the enzyme may offer some protection against S or serve in another metabolic role that has yet to be identified.

PubMed: 33776585
DOI: 10.1155/2021/8817136

実験手法

X-RAY DIFFRACTION (3.10003870577 Å)