6PEI

Structure of sorbitol dehydrogenase from Sinorhizobium meliloti 1021

6PEI の概要

• エントリーDOI: 10.2210/pdb6pei/pdb
• 分子名称: Sorbitol dehydrogenase (L-iditol 2-dehydrogenase) (2 entities in total)
• 機能のキーワード: smos, sorbitol, galactitol, dehydrogenase, oxidoreductase
• 由来する生物種: Sinorhizobium meliloti 1021 (Ensifer meliloti)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 123287.62

構造登録者

Bailey-Elkin, B.A.,Kohlmeier, M.G.,Oresnik, I.J.,Mark, B.L. (登録日: 2019-06-20, 公開日: 2020-06-24, 最終更新日: 2025-11-12)

主引用文献

Kohlmeier, M.G.,Bailey-Elkin, B.A.,Mark, B.L.,Oresnik, I.J.
Characterization of the sorbitol dehydrogenase SmoS from Sinorhizobium meliloti 1021.
Acta Crystallogr D Struct Biol, 77:380-390, 2021

PubMed Abstract: Sinorhizobium meliloti 1021 is a Gram-negative alphaproteobacterium with a robust capacity for carbohydrate metabolism. The enzymes that facilitate these reactions assist in the survival of the bacterium across a range of environmental niches, and they may also be suitable for use in industrial processes. SmoS is a dehydrogenase that catalyzes the oxidation of the commonly occurring sugar alcohols sorbitol and galactitol to fructose and tagatose, respectively, using NAD as a cofactor. The main objective of this study was to evaluate SmoS using biochemical techniques. The nucleotide sequence was codon-optimized for heterologous expression in Escherichia coli BL21 (DE3) Gold cells and the protein was subsequently overexpressed and purified. Size-exclusion chromatography and X-ray diffraction experiments suggest that SmoS is a tetramer. SmoS was crystallized, and crystals obtained in the absence of substrate diffracted to 2.1 Å resolution and those of a complex with sorbitol diffracted to 2.0 Å resolution. SmoS was characterized kinetically and shown to have a preference for sorbitol despite having a higher affinity for galactitol. Computational ligand-docking experiments suggest that tagatose binds the protein in a more energetically favourable complex than fructose, which is retained in the active site over a longer time frame following oxidation and reduces the rate of the reaction. These results supplement the inventory of biomolecules with potential for industrial applications and enhance the understanding of metabolism in the model organism S. meliloti.

PubMed: 33645541
DOI: 10.1107/S2059798321001017

実験手法

X-RAY DIFFRACTION (2.1 Å)