6PCX

Crystal Structure of a H5N1 influenza virus hemagglutinin at pH 6.0

6PCX の概要

• エントリーDOI: 10.2210/pdb6pcx/pdb
• 分子名称: Hemagglutinin, SULFATE ION, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: influenza, h5, viral protein
• 由来する生物種: Influenza A virus (A/mallard/Vietnam/3/2003(H5N1))
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 62150.09

構造登録者

Antanasijevic, A.,Durst, M.A.,Lavie, A.,Caffrey, M. (登録日: 2019-06-18, 公開日: 2019-11-13, 最終更新日: 2023-10-11)

主引用文献

Antanasijevic, A.,Durst, M.A.,Lavie, A.,Caffrey, M.
Identification of a pH sensor in Influenza hemagglutinin using X-ray crystallography.
J.Struct.Biol., 209:107412-107412, 2020

PubMed Abstract: Hemagglutnin (HA) mediates entry of influenza virus through a series of conformational changes triggered by the low pH of the endosome. The residue or combination of residues acting as pH sensors has not yet been fully elucidated. In this work, we assay pH effects on the structure of H5 HA by soaking HA crystallized at pH 6.5 in a series of buffers with lower pH, mimicking the conditions of the endosome. We find that HA1-H38, which is conserved in Group 1 HA, undergoes a striking change in side chain conformation, which we attribute to its protonation and cation-cation repulsion with conserved HA1-H18. This work suggests that x-ray crystallography can be applied for studying small-scale pH-induced conformational changes providing valuable information on the location of pH sensors in HA. Importantly, the observed change in HA1-H38 conformation is further evidence that the pH-induced conformational changes of HA are the result of a series of protonation events to conserved and non-conserved pH sensors.

PubMed: 31689502
DOI: 10.1016/j.jsb.2019.107412

実験手法

X-RAY DIFFRACTION (2.11 Å)