6PCP

Mechanism for regulation of DNA binding of Bordetella bronchiseptica BpsR by 6-hydroxynicotinic acid

6PCP の概要

• エントリーDOI: 10.2210/pdb6pcp/pdb
• 関連するPDBエントリー: 6PCO
• 分子名称: MarR family transcriptional regulator, 6-hydroxypyridine-3-carboxylic acid (3 entities in total)
• 機能のキーワード: inhibition, biofilm, transcription regulation, bordetella, bpsr, dna binding protein
• 由来する生物種: Bordetella pertussis
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 107198.82

構造登録者

Booth, W.T.,Davis, R.R.,Deora, R.,Hollis, T. (登録日: 2019-06-17, 公開日: 2019-11-06, 最終更新日: 2023-10-11)

主引用文献

Booth, W.T.,Davis, R.R.,Deora, R.,Hollis, T.
Structural mechanism for regulation of DNA binding of BpsR, a Bordetella regulator of biofilm formation, by 6-hydroxynicotinic acid.
Plos One, 14:e0223387-e0223387, 2019

PubMed Abstract: Bordetella bacteria are respiratory pathogens of humans, birds, and livestock. Bordetella pertussis the causative agent of whopping cough remains a significant health issue. The transcriptional regulator, BpsR, represses a number of Bordetella genes relating to virulence, cell adhesion, cell motility, and nicotinic acid metabolism. DNA binding of BpsR is allosterically regulated by interaction with 6-hydroxynicotinic acid (6HNA), the first product in the nicotinic acid degradation pathway. To understand the mechanism of this regulation, we have determined the crystal structures of BpsR and BpsR in complex with 6HNA. The structures reveal that BpsR binding of 6HNA induces a conformational change in the protein to prevent DNA binding. We have also identified homologs of BpsR in other Gram negative bacteria in which the amino acids involved in recognition of 6HNA are conserved, suggesting a similar mechanism for regulating nicotinic acid degradation.

PubMed: 31697703
DOI: 10.1371/journal.pone.0223387

実験手法

X-RAY DIFFRACTION (3.2 Å)