6PA0

Structure of the G77A mutant in Sodium Chloride

6PA0 の概要

• エントリーDOI: 10.2210/pdb6pa0/pdb
• 分子名称: Antibody HEAVY fragment, Antibody LIGHT fragment, pH-gated potassium channel KcsA, ... (7 entities in total)
• 機能のキーワード: potassium channel, kcsa, selectivity, permeation, protein transport, protein transport-immune system complex, protein transport/immune system
• 由来する生物種: Streptomyces lividans; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 58632.00

構造登録者

Cuello, L.G.,Guan, L. (登録日: 2019-06-11, 公開日: 2019-08-07, 最終更新日: 2024-10-23)

主引用文献

Tilegenova, C.,Cortes, D.M.,Jahovic, N.,Hardy, E.,Hariharan, P.,Guan, L.,Cuello, L.G.
Structure, function, and ion-binding properties of a K+channel stabilized in the 2,4-ion-bound configuration.
Proc.Natl.Acad.Sci.USA, 116:16829-16834, 2019

PubMed Abstract: Here, we present the atomic resolution crystallographic structure, the function, and the ion-binding properties of the KcsA mutants, G77A and G77C, that stabilize the 2,4-ion-bound configuration (i.e., water, K, water, K-ion-bound configuration) of the K channel's selectivity filter. A full functional and thermodynamic characterization of the G77A mutant revealed wild-type-like ion selectivity and apparent K-binding affinity, in addition to showing a lack of C-type inactivation gating and a marked reduction in its single-channel conductance. These structures validate, from a structural point of view, the notion that 2 isoenergetic ion-bound configurations coexist within a K channel's selectivity filter, which fully agrees with the water-K-ion-coupled transport detected by streaming potential measurements.

PubMed: 31387976
DOI: 10.1073/pnas.1901888116

実験手法

X-RAY DIFFRACTION (2.05 Å)