6P9W
Poliovirus (Type 1 Mahoney), receptor catalysed 135S particle map
Summary for 6P9W
| Entry DOI | 10.2210/pdb6p9w/pdb |
| EMDB information | 20275 20276 |
| Descriptor | VP1, VP2, VP3 (3 entities in total) |
| Functional Keywords | poliovirus, cell-entry intermediate, expanded virus, a-particle, virus |
| Biological source | Poliovirus type 1 (strain Mahoney) More |
| Total number of polymer chains | 3 |
| Total formula weight | 90111.88 |
| Authors | Hogle, J.M.,Filman, D.J.,Shah, P.N.M. (deposition date: 2019-06-10, release date: 2020-06-10, Last modification date: 2024-10-16) |
| Primary citation | Shah, P.N.M.,Filman, D.J.,Karunatilaka, K.S.,Hesketh, E.L.,Groppelli, E.,Strauss, M.,Hogle, J.M. Cryo-EM structures reveal two distinct conformational states in a picornavirus cell entry intermediate. Plos Pathog., 16:e1008920-e1008920, 2020 Cited by PubMed Abstract: The virions of enteroviruses such as poliovirus undergo a global conformational change after binding to the cellular receptor, characterized by a 4% expansion, and by the opening of holes at the two and quasi-three-fold symmetry axes of the capsid. The resultant particle is called a 135S particle or A-particle and is thought to be on the pathway to a productive infection. Previously published studies have concluded that the membrane-interactive peptides, namely VP4 and the N-terminus of VP1, are irreversibly externalized in the 135S particle. However, using established protocols to produce the 135S particle, and single particle cryo-electron microscopy methods, we have identified at least two unique states that we call the early and late 135S particle. Surprisingly, only in the "late" 135S particles have detectable levels of the VP1 N-terminus been trapped outside the capsid. Moreover, we observe a distinct density inside the capsid that can be accounted for by VP4 that remains associated with the genome. Taken together our results conclusively demonstrate that the 135S particle is not a unique conformation, but rather a family of conformations that could exist simultaneously. PubMed: 32997730DOI: 10.1371/journal.ppat.1008920 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.2 Å) |
Structure validation
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