6P5D

Photoactive Yellow Protein PYP 30ps

6P5D の概要

• エントリーDOI: 10.2210/pdb6p5d/pdb
• 分子名称: Photoactive yellow protein (2 entities in total)
• 機能のキーワード: pyp, 10ps, signaling protein
• 由来する生物種: Halorhodospira halophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14034.72

構造登録者

Pandey, S.,Schmidt, M. (登録日: 2019-05-30, 公開日: 2019-09-18, 最終更新日: 2023-11-15)

主引用文献

Pandey, S.,Bean, R.,Sato, T.,Poudyal, I.,Bielecki, J.,Cruz Villarreal, J.,Yefanov, O.,Mariani, V.,White, T.A.,Kupitz, C.,Hunter, M.,Abdellatif, M.H.,Bajt, S.,Bondar, V.,Echelmeier, A.,Doppler, D.,Emons, M.,Frank, M.,Fromme, R.,Gevorkov, Y.,Giovanetti, G.,Jiang, M.,Kim, D.,Kim, Y.,Kirkwood, H.,Klimovskaia, A.,Knoska, J.,Koua, F.H.M.,Letrun, R.,Lisova, S.,Maia, L.,Mazalova, V.,Meza, D.,Michelat, T.,Ourmazd, A.,Palmer, G.,Ramilli, M.,Schubert, R.,Schwander, P.,Silenzi, A.,Sztuk-Dambietz, J.,Tolstikova, A.,Chapman, H.N.,Ros, A.,Barty, A.,Fromme, P.,Mancuso, A.P.,Schmidt, M.
Time-resolved serial femtosecond crystallography at the European XFEL.
Nat.Methods, 17:73-78, 2020

PubMed Abstract: The European XFEL (EuXFEL) is a 3.4-km long X-ray source, which produces femtosecond, ultrabrilliant and spatially coherent X-ray pulses at megahertz (MHz) repetition rates. This X-ray source has been designed to enable the observation of ultrafast processes with near-atomic spatial resolution. Time-resolved crystallographic investigations on biological macromolecules belong to an important class of experiments that explore fundamental and functional structural displacements in these molecules. Due to the unusual MHz X-ray pulse structure at the EuXFEL, these experiments are challenging. Here, we demonstrate how a biological reaction can be followed on ultrafast timescales at the EuXFEL. We investigate the picosecond time range in the photocycle of photoactive yellow protein (PYP) with MHz X-ray pulse rates. We show that difference electron density maps of excellent quality can be obtained. The results connect the previously explored femtosecond PYP dynamics to timescales accessible at synchrotrons. This opens the door to a wide range of time-resolved studies at the EuXFEL.

PubMed: 31740816
DOI: 10.1038/s41592-019-0628-z

実験手法

X-RAY DIFFRACTION (1.6 Å)