6OWX

Spy H96L:Im7 L18pI-Phe complex; multiple anomalous datasets contained herein for element identification

6OWX の概要

• エントリーDOI: 10.2210/pdb6owx/pdb
• 分子名称: Periplasmic chaperone Spy, ZINC ION, CHLORIDE ION, ... (6 entities in total)
• 機能のキーワード: periplasmic, chaperone
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 24424.12

構造登録者

Rocchio, S.,Duman, R.,El Omari, K.,Mykhaylyk, V.,Yan, Z.,Wagner, A.,Bardwell, J.C.A.,Horowitz, S. (登録日: 2019-05-12, 公開日: 2019-05-29, 最終更新日: 2023-10-11)

主引用文献

Rocchio, S.,Duman, R.,El Omari, K.,Mykhaylyk, V.,Orr, C.,Yan, Z.,Salmon, L.,Wagner, A.,Bardwell, J.C.A.,Horowitz, S.
Identifying dynamic, partially occupied residues using anomalous scattering.
Acta Crystallogr D Struct Biol, 75:1084-1095, 2019

PubMed Abstract: Although often presented as taking single `snapshots' of the conformation of a protein, X-ray crystallography provides an averaged structure over time and space within the crystal. The important but difficult task of characterizing structural ensembles in crystals is typically limited to small conformational changes, such as multiple side-chain conformations. A crystallographic method was recently introduced that utilizes residual electron and anomalous density (READ) to characterize structural ensembles encompassing large-scale structural changes. Key to this method is an ability to accurately measure anomalous signals and distinguish them from noise or other anomalous scatterers. This report presents an optimized data-collection and analysis strategy for partially occupied iodine anomalous signals. Using the long-wavelength-optimized beamline I23 at Diamond Light Source, the ability to accurately distinguish the positions of anomalous scatterers with occupancies as low as ∼12% is demonstrated. The number and positions of these anomalous scatterers are consistent with previous biophysical, kinetic and structural data that suggest that the protein Im7 binds to the chaperone Spy in multiple partially occupied conformations. Finally, READ selections demonstrate that re-measured data using the new protocols are consistent with the previously characterized structural ensemble of the chaperone Spy with its client Im7. This study shows that a long-wavelength beamline results in easily validated anomalous signals that are strong enough to be used to detect and characterize highly disordered sections of crystal structures.

PubMed: 31793902
DOI: 10.1107/S2059798319014475

実験手法

X-RAY DIFFRACTION (2.06 Å)