6OWS

Cryo-EM structure of an Acinetobacter baumannii multidrug efflux pump

6OWS の概要

• エントリーDOI: 10.2210/pdb6ows/pdb
• EMDBエントリー: 20216
• 分子名称: Efflux pump membrane transporter, PHOSPHATIDYLETHANOLAMINE (2 entities in total)
• 機能のキーワード: transporter, membrane protein
• 由来する生物種: Acinetobacter baumannii
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 342169.13

構造登録者

Su, C.-C. (登録日: 2019-05-10, 公開日: 2019-06-19, 最終更新日: 2025-06-04)

主引用文献

Su, C.C.,Morgan, C.E.,Kambakam, S.,Rajavel, M.,Scott, H.,Huang, W.,Emerson, C.C.,Taylor, D.J.,Stewart, P.L.,Bonomo, R.A.,Yu, E.W.
Cryo-Electron Microscopy Structure of an Acinetobacter baumannii Multidrug Efflux Pump.
Mbio, 10:-, 2019

PubMed Abstract: Resistance-nodulation-cell division multidrug efflux pumps are membrane proteins that catalyze the export of drugs and toxic compounds out of bacterial cells. Within the hydrophobe-amphiphile subfamily, these multidrug-resistant proteins form trimeric efflux pumps. The drug efflux process is energized by the influx of protons. Here, we use single-particle cryo-electron microscopy to elucidate the structure of the AdeB multidrug efflux pump embedded in lipidic nanodiscs to a resolution of 2.98 Å. We found that each AdeB molecule within the trimer preferentially takes the resting conformational state in the absence of substrates. We propose that proton influx and drug efflux are synchronized and coordinated within the transport cycle. is a successful human pathogen which has emerged as one of the most problematic and highly antibiotic-resistant Gram-negative bacteria worldwide. Multidrug efflux is a major mechanism that uses to counteract the action of multiple classes of antibiotics, such as β-lactams, tetracyclines, fluoroquinolones, and aminoglycosides. Here, we report a cryo-electron microscopy (cryo-EM) structure of the prevalent AdeB multidrug efflux pump, which indicates a plausible pathway for multidrug extrusion. Overall, our data suggest a mechanism for energy coupling that powers up this membrane protein to export antibiotics from bacterial cells. Our studies will ultimately inform an era in structure-guided drug design to combat multidrug resistance in these Gram-negative pathogens.

PubMed: 31266873
DOI: 10.1128/mBio.01295-19

実験手法

ELECTRON MICROSCOPY (2.98 Å)