6OWF

Structure of a synthetic beta-carboxysome shell, T=3

これはPDB形式変換不可エントリーです。

6OWF の概要

• エントリーDOI: 10.2210/pdb6owf/pdb
• EMDBエントリー: 20208
• 分子名称: Microcompartments protein, Ethanolamine utilization protein EutN/carboxysome structural protein Ccml (2 entities in total)
• 機能のキーワード: bacterial microcompartments, carboxysome, structural protein
• 由来する生物種: Halothece sp. (strain PCC 7418); 詳細
• タンパク質・核酸の鎖数: 180
• 化学式量合計: 2150219.40

構造登録者

Sutter, M.,Laughlin, T.G.,Davies, K.M.,Kerfeld, C.A. (登録日: 2019-05-09, 公開日: 2019-09-25, 最終更新日: 2024-03-13)

主引用文献

Sutter, M.,Laughlin, T.G.,Sloan, N.B.,Serwas, D.,Davies, K.M.,Kerfeld, C.A.
Structure of a Syntheticbeta-Carboxysome Shell.
Plant Physiol., 181:1050-1058, 2019

PubMed Abstract: Carboxysomes are capsid-like, CO-fixing organelles that are present in all cyanobacteria and some chemoautotrophs and that substantially contribute to global primary production. They are composed of a selectively permeable protein shell that encapsulates Rubisco, the principal CO-fixing enzyme, and carbonic anhydrase. As the centerpiece of the carbon-concentrating mechanism, by packaging enzymes that collectively enhance catalysis, the carboxysome shell enables the generation of a locally elevated concentration of substrate CO and the prevention of CO escape. A functional carboxysome consisting of an intact shell and cargo is essential for cyanobacterial growth under ambient CO concentrations. Using cryo-electron microscopy, we have determined the structure of a recombinantly produced simplified β-carboxysome shell. The structure reveals the sidedness and the specific interactions between the carboxysome shell proteins. The model provides insight into the structural basis of selective permeability of the carboxysome shell and can be used to design modifications to investigate the mechanisms of cargo encapsulation and other physiochemical properties such as permeability. Notably, the permeability properties are of great interest for modeling and evaluating this carbon-concentrating mechanism in metabolic engineering. Moreover, we find striking similarity between the carboxysome shell and the structurally characterized, evolutionarily distant metabolosome shell, implying universal architectural principles for bacterial microcompartment shells.

PubMed: 31501298
DOI: 10.1104/pp.19.00885

実験手法

ELECTRON MICROSCOPY (3 Å)