6OV8

2.6 Angstrom Resolution Crystal Structure of Aminopeptidase B from Escherichia coli str. K-12 substr. MG1655

6OV8 の概要

• エントリーDOI: 10.2210/pdb6ov8/pdb
• 関連するPDBエントリー: 6OAD
• 分子名称: Peptidase B, ZINC ION, MANGANESE (II) ION, ... (5 entities in total)
• 機能のキーワード: structural genomics, center for structural genomics of infectious diseases, csgid, aminopeptidase b, hydrolase
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 283012.54

構造登録者

Minasov, G.,Shuvalova, L.,Wawrzak, Z.,Kiryukhina, O.,Grimshaw, S.,Kwon, K.,Satchell, K.J.F.,Center for Structural Genomics of Infectious Diseases (CSGID) (登録日: 2019-05-07, 公開日: 2019-05-15, 最終更新日: 2024-11-13)

主引用文献

Minasov, G.,Lam, M.R.,Rosas-Lemus, M.,Slawek, J.,Woinska, M.,Shabalin, I.G.,Shuvalova, L.,Palsson, B.O.,Godzik, A.,Minor, W.,Satchell, K.J.F.
Comparison of metal-bound and unbound structures of aminopeptidase B proteins from Escherichia coli and Yersinia pestis.
Protein Sci., 29:1618-1628, 2020

PubMed Abstract: Protein degradation by aminopeptidases is involved in bacterial responses to stress. Escherichia coli produces two metal-dependent M17 family leucine aminopeptidases (LAPs), aminopeptidase A (PepA) and aminopeptidase B (PepB). Several structures have been solved for PepA as well as other bacterial M17 peptidases. Herein, we report the first structures of a PepB M17 peptidase. The E. coli PepB protein structure was determined at a resolution of 2.05 and 2.6 Å. One structure has both Zn and Mn , while the second structure has two Zn ions bound to the active site. A 2.75 Å apo structure is also reported for PepB from Yersinia pestis. Both proteins form homohexamers, similar to the overall arrangement of PepA and other M17 peptidases. However, the divergent N-terminal domain in PepB is much larger resulting in a tertiary structure that is more expanded. Modeling of a dipeptide substrate into the C-terminal LAP domain reveals contacts that account for PepB to uniquely cleave after aspartate.

PubMed: 32306515
DOI: 10.1002/pro.3876

実験手法

X-RAY DIFFRACTION (2.61 Å)