6OV2

Crystal structure of human claudin-9 in complex with Clostridium perfringens entertoxin C-terminal domain in closed form

6OV2 の概要

• エントリーDOI: 10.2210/pdb6ov2/pdb
• 関連するPDBエントリー: 6OV3
• 分子名称: Claudin-9, Heat-labile enterotoxin B chain, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: claudin, enterotoxin, tight junction protein, transmembrane protein, cell adhesion
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37749.87

構造登録者

Vecchio, A.J.,Stroud, R.M. (登録日: 2019-05-06, 公開日: 2019-09-04, 最終更新日: 2024-10-16)

主引用文献

Vecchio, A.J.,Stroud, R.M.
Claudin-9 structures reveal mechanism for toxin-induced gut barrier breakdown.
Proc.Natl.Acad.Sci.USA, 116:17817-17824, 2019

PubMed Abstract: The human pathogenic bacterium secretes an enterotoxin (CpE) that targets claudins through its C-terminal receptor-binding domain (cCpE). Isoform-specific binding by CpE causes dissociation of claudins and tight junctions (TJs), resulting in cytotoxicity and breakdown of the gut epithelial barrier. Here, we present crystal structures of human claudin-9 (hCLDN-9) in complex with cCpE at 3.2 and 3.3 Å. We show that hCLDN-9 is a high-affinity CpE receptor and that hCLDN-9-expressing cells undergo cell death when treated with CpE but not cCpE, which lacks its cytotoxic domain. Structures reveal cCpE-induced alterations to 2 epitopes known to enable claudin self-assembly and expose high-affinity interactions between hCLDN-9 and cCpE that explain isoform-specific recognition. These findings elucidate the molecular bases for hCLDN-9 selective ion permeability and binding by CpE, and provide mechanisms for how CpE disrupts gut homeostasis by dissociating claudins and TJs to affect epithelial adhesion and intercellular transport.

PubMed: 31434788
DOI: 10.1073/pnas.1908929116

実験手法

X-RAY DIFFRACTION (3.2 Å)