6OPF

Crystal structure of dmNxf2 UBA domain fused with Panoramix helix

6OPF の概要

• エントリーDOI: 10.2210/pdb6opf/pdb
• 関連するPDBエントリー: 6MRK
• 分子名称: Nuclear RNA export factor 2, Panoramix fusion (2 entities in total)
• 機能のキーワード: piwi, transposon silencing, heterochromatin formation, pirna pathway, transcriptional silencing, rna binding protein
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 44301.93

構造登録者

Wang, J.,Patel, D.J. (登録日: 2019-04-24, 公開日: 2019-07-03, 最終更新日: 2023-10-11)

主引用文献

Batki, J.,Schnabl, J.,Wang, J.,Handler, D.,Andreev, V.I.,Stieger, C.E.,Novatchkova, M.,Lampersberger, L.,Kauneckaite, K.,Xie, W.,Mechtler, K.,Patel, D.J.,Brennecke, J.
The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation.
Nat.Struct.Mol.Biol., 26:720-731, 2019

PubMed Abstract: The PIWI-interacting RNA (piRNA) pathway protects genome integrity in part through establishing repressive heterochromatin at transposon loci. Silencing requires piRNA-guided targeting of nuclear PIWI proteins to nascent transposon transcripts, yet the subsequent molecular events are not understood. Here, we identify SFiNX (silencing factor interacting nuclear export variant), an interdependent protein complex required for Piwi-mediated cotranscriptional silencing in Drosophila. SFiNX consists of Nxf2-Nxt1, a gonad-specific variant of the heterodimeric messenger RNA export receptor Nxf1-Nxt1 and the Piwi-associated protein Panoramix. SFiNX mutant flies are sterile and exhibit transposon derepression because piRNA-loaded Piwi is unable to establish heterochromatin. Within SFiNX, Panoramix recruits heterochromatin effectors, while the RNA binding protein Nxf2 licenses cotranscriptional silencing. Our data reveal how Nxf2 might have evolved from an RNA transport receptor into a cotranscriptional silencing factor. Thus, NXF variants, which are abundant in metazoans, can have diverse molecular functions and might have been coopted for host genome defense more broadly.

PubMed: 31384064
DOI: 10.1038/s41594-019-0270-6

実験手法

X-RAY DIFFRACTION (2 Å)