6OMT

HIV-1 capsid hexamer R18D mutant

6OMT の概要

• エントリーDOI: 10.2210/pdb6omt/pdb
• 分子名称: Capsid protein (2 entities in total)
• 機能のキーワード: hiv-1, capsid, ca, viral protein
• 由来する生物種: Human immunodeficiency virus 1 (HIV-1)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25550.36

構造登録者

Huang, P.,Summers, B.J.,Xiong, Y. (登録日: 2019-04-19, 公開日: 2019-08-21, 最終更新日: 2024-11-20)

主引用文献

Huang, P.T.,Summers, B.J.,Xu, C.,Perilla, J.R.,Malikov, V.,Naghavi, M.H.,Xiong, Y.
FEZ1 Is Recruited to a Conserved Cofactor Site on Capsid to Promote HIV-1 Trafficking.
Cell Rep, 28:2373-, 2019

PubMed Abstract: HIV-1 uses the microtubule network to traffic the viral capsid core toward the nucleus. Viral nuclear trafficking and infectivity require the kinesin-1 adaptor protein FEZ1. Here, we demonstrate that FEZ1 directly interacts with the HIV-1 capsid and specifically binds capsid protein (CA) hexamers. FEZ1 contains multiple acidic, poly-glutamate stretches that interact with the positively charged central pore of CA hexamers. The FEZ1-capsid interaction directly competes with nucleotides and inositol hexaphosphate (IP6) that bind at the same location. In addition, all-atom molecular dynamic (MD) simulations establish the molecular details of FEZ1-capsid interactions. Functionally, mutation of the FEZ1 capsid-interacting residues significantly reduces trafficking of HIV-1 particles toward the nucleus and early infection. These findings support a model in which the central capsid hexamer pore is a general HIV-1 cofactor-binding hub and FEZ1 serves as a unique CA hexamer pattern sensor to recognize this site and promote capsid trafficking in the cell.

PubMed: 31422020
DOI: 10.1016/j.celrep.2019.07.079

実験手法

X-RAY DIFFRACTION (2.052 Å)