6OL5

Structure of iglb12 Fab in complex with anti-idiotype ib3 Fab

6OL5 の概要

• エントリーDOI: 10.2210/pdb6ol5/pdb
• 分子名称: ib3 Heavy Chain, ib3 Light Chain, iglb12 Heavy Chain, ... (9 entities in total)
• 機能のキーワード: glb12, antibody, ib3, fab, complex, anti-idiotype, immune system, hiv, broadly neutralizing
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 97879.72

構造登録者

Weidle, C.,Pancera, M. (登録日: 2019-04-15, 公開日: 2019-07-24, 最終更新日: 2024-10-09)

主引用文献

Bancroft, T.,DeBuysscher, B.L.,Weidle, C.,Schwartz, A.,Wall, A.,Gray, M.D.,Feng, J.,Steach, H.R.,Fitzpatrick, K.S.,Gewe, M.M.,Skog, P.D.,Doyle-Cooper, C.,Ota, T.,Strong, R.K.,Nemazee, D.,Pancera, M.,Stamatatos, L.,McGuire, A.T.,Taylor, J.J.
Detection and activation of HIV broadly neutralizing antibody precursor B cells using anti-idiotypes.
J.Exp.Med., 216:2331-2347, 2019

PubMed Abstract: Many tested vaccines fail to provide protection against disease despite the induction of antibodies that bind the pathogen of interest. In light of this, there is much interest in rationally designed subunit vaccines that direct the antibody response to protective epitopes. Here, we produced a panel of anti-idiotype antibodies able to specifically recognize the inferred germline version of the human immunodeficiency virus 1 (HIV-1) broadly neutralizing antibody b12 (iglb12). We determined the crystal structure of two anti-idiotypes in complex with iglb12 and used these anti-idiotypes to identify rare naive human B cells expressing B cell receptors with similarity to iglb12. Immunization with a multimerized version of this anti-idiotype induced the proliferation of transgenic murine B cells expressing the iglb12 heavy chain in vivo, despite the presence of deletion and anergy within this population. Together, our data indicate that anti-idiotypes are a valuable tool for the study and induction of potentially protective antibodies.

PubMed: 31345930
DOI: 10.1084/jem.20190164

実験手法

X-RAY DIFFRACTION (2.9 Å)