6OL3

Crystal structure of an adenovirus virus-associated RNA

Summary for 6OL3

• Entry DOI: 10.2210/pdb6ol3/pdb
• Descriptor: Adenovirus Virus-Associated (VA) RNA I apical and central domains, POTASSIUM ION (3 entities in total)
• Functional Keywords: noncoding rna, viral rna, rna
• Biological source: Human adenovirus 2
• Total number of polymer chains: 1
• Total formula weight: 36161.57

Authors

Hood, I.V.,Gordon, J.M.,Bou-Nader, C.,Henderson, F.V.,Bahmanjah, S.,Zhang, J. (deposition date: 2019-04-15, release date: 2019-07-03, Last modification date: 2024-03-13)

Primary citation

Hood, I.V.,Gordon, J.M.,Bou-Nader, C.,Henderson, F.E.,Bahmanjah, S.,Zhang, J.
Crystal structure of an adenovirus virus-associated RNA.
Nat Commun, 10:2871-2871, 2019

PubMed Abstract: Adenovirus Virus-Associated (VA) RNAs are the first discovered viral noncoding RNAs. By mimicking double-stranded RNAs (dsRNAs), the exceptionally abundant, multifunctional VA RNAs sabotage host machineries that sense, transport, process, or edit dsRNAs. How VA-I suppresses PKR activation despite its strong dsRNA character, and inhibits the crucial antiviral kinase to promote viral translation, remains largely unknown. Here, we report a 2.7 Å crystal structure of VA-I RNA. The acutely bent VA-I features an unusually structured apical loop, a wobble-enriched, coaxially stacked apical and tetra-stems necessary and sufficient for PKR inhibition, and a central domain pseudoknot that resembles codon-anticodon interactions and prevents PKR activation by VA-I. These global and local structural features collectively define VA-I as an archetypal PKR inhibitor made of RNA. The study provides molecular insights into how viruses circumnavigate cellular rules of self vs non-self RNAs to not only escape, but further compromise host innate immunity.

PubMed: 31253805
DOI: 10.1038/s41467-019-10752-6

Experimental method

X-RAY DIFFRACTION (2.74 Å)