6OKW

Solution structure of VEK50

6OKW の概要

• エントリーDOI: 10.2210/pdb6okw/pdb
• NMR情報: BMRB: 30391
• 分子名称: Plasminogen-binding group A streptococcal M-like protein PAM (1 entity in total)
• 機能のキーワード: plasminogen binding peptide, protein binding
• 由来する生物種: Streptococcus pyogenes
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6173.79

構造登録者

Yuan, Y.,Castellino, F.J. (登録日: 2019-04-15, 公開日: 2020-02-26, 最終更新日: 2024-05-15)

主引用文献

Yuan, Y.,Ayinuola, Y.A.,Singh, D.,Ayinuola, O.,Mayfield, J.A.,Quek, A.,Whisstock, J.C.,Law, R.H.P.,Lee, S.W.,Ploplis, V.A.,Castellino, F.J.
Solution structural model of the complex of the binding regions of human plasminogen with its M-protein receptor from Streptococcus pyogenes.
J.Struct.Biol., 208:18-29, 2019

PubMed Abstract: VEK50 is a truncated peptide from a Streptococcal pyogenes surface human plasminogen (hPg) binding M-protein (PAM). VEK50 contains the full A-domain of PAM, which is responsible for its low nanomolar binding to hPg. The interaction of VEK50 with kringle 2, the PAM-binding domain in hPg (K2), has been studied by high-resolution NMR spectroscopy. The data show that each VEK50 monomer in solution contains two tight binding sites for K2, one each in the a1- (RH1; RH) and a2- (RH2; RH) repeats within the A-domain of VEK50. Two mutant forms of VEK50, viz., VEK50[RH1/AA] (VEK50) and VEK50[RH2/AA] (VEK50), were designed by replacing each RH with AA, thus eliminating one of the K2 binding sites within VEK50, and allowing separate study of each binding site. Using C- and N-labeled peptides, NMR-derived solution structures of VEK50 in its complex with K2 were solved. We conclude that the A-domain of PAM can accommodate two molecules of K2 docked within a short distance of each other, and the strength of the binding is slightly different for each site. The solution structure of the VEK50/K2, complex, which is a reductionist model of the PAM/hPg complex, provides insights for the binding mechanism of PAM to a host protein, a process that is critical to S. pyogenes virulence.

PubMed: 31301349
DOI: 10.1016/j.jsb.2019.07.005

実験手法

SOLUTION NMR