6OF2

Precursor ribosomal RNA processing complex, State 2.

6OF2 の概要

• エントリーDOI: 10.2210/pdb6of2/pdb
• EMDBエントリー: 20040
• 分子名称: Ribonuclease, CLP1_P domain-containing protein, PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER, ... (4 entities in total)
• 機能のキーワード: complex, ribonuclease, polynucleotide kinase, rna binding protein
• 由来する生物種: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 228101.92

構造登録者

Pillon, M.C.,Hsu, A.L.,Krahn, J.M.,Williams, J.G.,Goslen, K.H.,Sobhany, M.,Borgnia, M.J.,Stanley, R.E. (登録日: 2019-03-28, 公開日: 2019-09-11, 最終更新日: 2025-06-04)

主引用文献

Pillon, M.C.,Hsu, A.L.,Krahn, J.M.,Williams, J.G.,Goslen, K.H.,Sobhany, M.,Borgnia, M.J.,Stanley, R.E.
Cryo-EM reveals active site coordination within a multienzyme pre-rRNA processing complex.
Nat.Struct.Mol.Biol., 26:830-839, 2019

PubMed Abstract: Ribosome assembly is a complex process reliant on the coordination of trans-acting enzymes to produce functional ribosomal subunits and secure the translational capacity of cells. The endoribonuclease (RNase) Las1 and the polynucleotide kinase (PNK) Grc3 assemble into a multienzyme complex, herein designated RNase PNK, to orchestrate processing of precursor ribosomal RNA (rRNA). RNase PNK belongs to the functionally diverse HEPN nuclease superfamily, whose members rely on distinct cues for nuclease activation. To establish how RNase PNK coordinates its dual enzymatic activities, we solved a series of cryo-EM structures of Chaetomium thermophilum RNase PNK in multiple conformational states. The structures reveal that RNase PNK adopts a butterfly-like architecture, harboring a composite HEPN nuclease active site flanked by discrete RNA kinase sites. We identify two molecular switches that coordinate nuclease and kinase function. Together, our structures and corresponding functional studies establish a new mechanism of HEPN nuclease activation essential for ribosome production.

PubMed: 31488907
DOI: 10.1038/s41594-019-0289-8

実験手法

ELECTRON MICROSCOPY (2.9 Å)