6OEE

Structure of CagT from a cryo-EM reconstruction of a T4SS

6OEE の概要

• エントリーDOI: 10.2210/pdb6oee/pdb
• EMDBエントリー: 20020
• 分子名称: Type IV secretion system apparatus protein CagT (1 entity in total)
• 機能のキーワード: t4ss, secretion, h. pylori, translocase
• 由来する生物種: Helicobacter pylori
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 453865.59

構造登録者

Chung, J.M.,Sheedlo, M.J.,Campbell, A.,Sawhney, N.,Frick-Cheng, A.E.,Lacy, D.B.,Cover, T.L.,Ohi, M.D. (登録日: 2019-03-27, 公開日: 2019-07-03, 最終更新日: 2024-03-13)

主引用文献

Chung, J.M.,Sheedlo, M.J.,Campbell, A.M.,Sawhney, N.,Frick-Cheng, A.E.,Lacy, D.B.,Cover, T.L.,Ohi, M.D.
Structure of the Helicobacter pylori Cag type IV secretion system.
Elife, 8:-, 2019

PubMed Abstract: Bacterial type IV secretion systems (T4SSs) are molecular machines that can mediate interbacterial DNA transfer through conjugation and delivery of effector molecules into host cells. The Cag T4SS translocates CagA, a bacterial oncoprotein, into gastric cells, contributing to gastric cancer pathogenesis. We report the structure of a membrane-spanning Cag T4SS assembly, which we describe as three sub-assemblies: a 14-fold symmetric outer membrane core complex (OMCC), 17-fold symmetric periplasmic ring complex (PRC), and central stalk. Features that differ markedly from those of prototypical T4SSs include an expanded OMCC and unexpected symmetry mismatch between the OMCC and PRC. This structure is one of the largest bacterial secretion system assemblies ever reported and illustrates the remarkable structural diversity that exists among bacterial T4SSs.

PubMed: 31210639
DOI: 10.7554/eLife.47644

実験手法

ELECTRON MICROSCOPY (3.8 Å)