6OE3

Crystal Structure of HIV-1 Reverse Transcriptase in Complex with 5-(2-(2-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)ethoxy)phenoxy)-7-fluoro-2-naphthonitrile (JLJ635), a Non-nucleoside Inhibitor

6OE3 の概要

• エントリーDOI: 10.2210/pdb6oe3/pdb
• 分子名称: HIV-1 REVERSE TRANSCRIPTASE, P66 SUBUNIT, HIV-1 REVERSE TRANSCRIPTASE, P51 SUBUNIT, 5-{2-[2-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)ethoxy]phenoxy}-7-fluoronaphthalene-2-carbonitrile, ... (6 entities in total)
• 機能のキーワード: nnrtis, hiv-1, drug design, transferase
• 由来する生物種: Human immunodeficiency virus type 1 group M subtype B (isolate BH10); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 114566.48

構造登録者

Bertoletti, N.,Kudalkar, S.N.,Anderson, K.S.,Cisneros Trigo, J.A.,Jorgensen, W.L. (登録日: 2019-03-27, 公開日: 2019-05-15, 最終更新日: 2023-10-11)

主引用文献

Kudalkar, S.N.,Ullah, I.,Bertoletti, N.,Mandl, H.K.,Cisneros, J.A.,Beloor, J.,Chan, A.H.,Quijano, E.,Saltzman, W.M.,Jorgensen, W.L.,Kumar, P.,Anderson, K.S.
Structural and pharmacological evaluation of a novel non-nucleoside reverse transcriptase inhibitor as a promising long acting nanoformulation for treating HIV.
Antiviral Res., 167:110-116, 2019

PubMed Abstract: Combination antiretroviral therapy (cART) has been proven effective in inhibiting human immunodeficiency virus type 1 (HIV-1) infection and has significantly improved the health outcomes in acquired immune deficiency syndrome (AIDS) patients. The therapeutic benefits of cART have been challenged because of the toxicity and emergence of drug-resistant HIV-1 strains along with lifelong patient compliance resulting in non-adherence. These issues also hinder the clinical benefits of non-nucleoside reverse transcriptase inhibitors (NNRTIs), which are one of the vital components of cART for the treatment of HIV-1 infection. In this study, using a computational and structural based drug design approach, we have discovered an effective HIV -1 NNRTI, compound I (Cmpd I) that is very potent in biochemical assays and which targets key residues in the allosteric binding pocket of wild-type (WT)-RT as revealed by structural studies. Furthermore, Cmpd I exhibited very potent antiviral activity in HIV-1 infected T cells, lacked cytotoxicity (therapeutic index >100,000), and no significant off-target effects were noted in pharmacological assays. To address the issue of non-adherence, we developed a long-acting nanoformulation of Cmpd I (Cmpd I-NP) using poly (lactide-coglycolide) (PLGA) particles. The pharmacokinetic studies of free and nanoformulated Cmpd I were carried out in BALB/c mice. Intraperitoneal administration of Cmpd I and Cmpd I-NP in BALB/c mice revealed prolonged serum residence time of 48 h and 30 days, respectively. The observed serum concentrations of Cmpd I in both cases were sufficient to provide >97% inhibition in HIV-1 infected T-cells. The significant antiviral activity along with favorable pharmacological and pharmacokinetic profile of Cmpd I, provide compelling and critical support for its further development as an anti-HIV therapeutic agent.

PubMed: 31034849
DOI: 10.1016/j.antiviral.2019.04.010

実験手法

X-RAY DIFFRACTION (2.9 Å)