6OD9

Co-crystal structure of the Fusobacterium ulcerans ZTP riboswitch using an X-ray free-electron laser

6OD9 の概要

• エントリーDOI: 10.2210/pdb6od9/pdb
• 関連するPDBエントリー: 5BTP
• 分子名称: RNA (75-MER), MAGNESIUM ION, AMINOIMIDAZOLE 4-CARBOXAMIDE RIBONUCLEOTIDE, ... (6 entities in total)
• 機能のキーワード: complex, riboswitch, x-ray free-electron diffraction, xfel, zmp, ztp, rna
• 由来する生物種: Fusobacterium ulcerans
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49581.20

構造登録者

Jones, C.P.,Tran, B.,Ferre-D'Amare, A.R. (登録日: 2019-03-26, 公開日: 2019-07-17, 最終更新日: 2023-10-11)

主引用文献

Jones, C.,Tran, B.,Conrad, C.,Stagno, J.,Trachman 3rd, R.,Fischer, P.,Meents, A.,Ferre-D'Amare, A.
Co-crystal structure of the Fusobacterium ulcerans ZTP riboswitch using an X-ray free-electron laser.
Acta Crystallogr.,Sect.F, 75:496-500, 2019

PubMed Abstract: Riboswitches are conformationally dynamic RNAs that regulate gene expression by binding specific small molecules. ZTP riboswitches bind the purine-biosynthetic intermediate 5-aminoimidazole-4-carboxamide riboside 5'-monophosphate (ZMP) and its triphosphorylated form (ZTP). Ligand binding to this riboswitch ultimately upregulates genes involved in folate and purine metabolism. Using an X-ray free-electron laser (XFEL), the room-temperature structure of the Fusobacterium ulcerans ZTP riboswitch bound to ZMP has now been determined at 4.1 Å resolution. This model, which was refined against a data set from ∼750 diffraction images (each from a single crystal), was found to be consistent with that previously obtained from data collected at 100 K using conventional synchrotron X-radiation. These experiments demonstrate the feasibility of time-resolved XFEL experiments to understand how the ZTP riboswitch accommodates cognate ligand binding.

PubMed: 31282869
DOI: 10.1107/S2053230X19008549

実験手法

X-RAY DIFFRACTION (4.102 Å)