6OCI
Crystal Structure of Equine Serum Albumin in Complex with Ibuprofen
Summary for 6OCI
| Entry DOI | 10.2210/pdb6oci/pdb |
| Descriptor | Serum albumin, IBUPROFEN, SUCCINIC ACID, ... (5 entities in total) |
| Functional Keywords | helical, three-domain protein, serum albumin, transport protein |
| Biological source | Equus caballus (Horse) |
| Total number of polymer chains | 1 |
| Total formula weight | 66530.77 |
| Authors | Sekula, B.,Zielinski, K.,Bujacz, A.,Bujacz, G. (deposition date: 2019-03-24, release date: 2020-01-15, Last modification date: 2024-10-23) |
| Primary citation | Zielinski, K.,Sekula, B.,Bujacz, A.,Szymczak, I. Structural investigations of stereoselective profen binding by equine and leporine serum albumins. Chirality, 32:334-344, 2020 Cited by PubMed Abstract: Serum albumin, the most abundant transport protein of mammalian blood, interacts with various nonsteroidal anti-inflammatory drugs (NSAIDs) affecting their disposition, metabolism, and excretion. A big group of chiral NSAIDs transported by albumin, profens, is created by derivatives of 2-arylpropionic acid. The chiral center in the structures of profens is adjacent to the carboxylate moiety and often determines different pharmacological properties of profen enantiomers. This study describes crystal structures of two albumins, isolated from equine and leporine serum, in complexes with three profens: ibuprofen, ketoprofen, and suprofen. Based on three-dimensional structures, the stereoselectivity of albumin is discussed and referred to the previously published albumin complexes with drugs. Drug Site 2 (DS2) of albumin, the bulky hydrophobic pocket of subdomain IIIA with a patch of polar residues, preferentially binds (S)-enantiomers of all investigated profens. Almost identical binding mode of all these drugs clearly indicates the stereoselectivity of DS2 towards (S)-profens in different albumin species. Also, the affinity studies show that DS2 is the major site that presents high affinity towards investigated drugs. Additionally, crystallographic data reveal the secondary binding sites of ketoprofen in leporine serum albumin and ibuprofen in equine serum albumin, both overlapping with previously identified naproxen binding sites: the cleft formed between subdomains IIIA and IIIB close to the fatty acid binding site 5 and the niche created between subdomains IIA and IIIA, called fatty acid site 6. PubMed: 31905261DOI: 10.1002/chir.23162 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.54 Å) |
Structure validation
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